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  • 1
    ISSN: 1432-1432
    Keywords: Repetitive DNA ; Tandem repeats ; Sequence analysis ; Recombination ; Isolated populations ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The satellite DNA family pDoP102 is species specific for the cave cricket Dolichopoda schiavazzii, an endemic species of mainland and insular Tuscany. It consists of numerous tandemly arranged repeats, 102 bp in length, and evolved most probably after cladogenesis of D. schiavazzii from the D. baccettii-aegilion group within the last 2.3 ± 0.8 million years. A sequence comparison of 31 clones (53 repetition units) from three isolated populations reveals a very high degree of sequence homogeneity within the species with no evidence for any specific population features. This appears to be in contrast to the results of allozyme analyses which account for a relatively old evolutionary divergence of the Elba island population from the mainland ones. Since the assumption of actual gene flow and recent colonization is rejected, the observed sequence homogeneity is hypothesized to be maintained by recombination processes preventing fixation of newly introduced mutations on pDoP102 sequence clusters.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1432
    Keywords: Concerted evolution ; Noncoding DNA ; Recombination ; Sequence homogenization ; Unequal crossing-over
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three specific satellite DNA families can be detected in the genome of the cave cricketDolichopoda schiavazzii. ThepDoP102 and thepDsPv400 families are species specific forD. schiavazzii; thepDoP500 family is probably present in allDolichopoda species. The three satellite DNA families were characterized from individuals of three isolated populations ofD. schiavazzii with respect to nucleotide sequence, sequence complexity, sequence variability, and copy number. This unique data set on satellite DNAs of D. schiavazzii seems to allow one to test the significance of theoretical approaches to the mode of evolution of noncoding, tandemly arranged satellite DNA. At least for satellite DNAs ofD. schiavazzii two clear trends were observed: (1) sequence variability increases with copy number and (2) the repeat length decreases with copy number. The first trend is in good agreement with the theory but the second is not. Thus, a revision of the models is proposed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1432
    Keywords: Highly repetitive DNA ; DNA evolution ; Genome evolution ; Sequence analysis ; Heterochromatic sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pvB370 BamHI tandemly repeated satellite DNA family was isolated and analyzed in eight species of the Drosophila virilis group and is probably common to all its members. Unexpectedly, the satellite DNA family showed similar levels of intra- and interspecific sequence variability. An EMBL gene-bank search revealed a high degree of similarity between the members of the pvB370 BamHI satellite DNA sequence family and the direct terminal repeats of the mobile dispersed genetic pDv elements described in D. virilis and other species of the group. However, no similarity was observed to the transcribed and translated part of the pDv elements. It is suggested that the satellite DNA repeats are phylogenetically older than the mobile dispersed genetic pDv element and that the functional pDv elements might have derived from the satellite DNA family through an insertion of a tandemly repeated 36-bp transcription unit.
    Type of Medium: Electronic Resource
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