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  • Atlantic Ocean; Calculated using AquaEnv R package; Calculated using respR R package; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Oxygen, dissolved; Oxygen saturation; Oxygen sensor, Atlas Scientific Lab grade; pH; pH sensor, Atlas Scientific, lab grade; Temperature, water; Thermometer, PT100; Type of study  (1)
  • Atlantic Ocean; Comment; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; Flow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R); flow cytometry; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Prokaryotes; prokaryotic abundance; Replicate; Species; Species, unique identification (Semantic URI); Species, unique identification (URI); Type of study  (1)
  • Brain biopsy  (1)
Document type
Keywords
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  • 1
    ISSN: 1432-1459
    Keywords: Key words AIDS ; Brain biopsy ; Cerebral spinal fluid ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Twenty patients with AIDS who had intracranial lesions underwent both brain biopsy and cerebro-spinal fluid (CSF) examination to compare histological diagnosis with the polymerase chain reaction (CSF-PCR) for the identification of infectious agents. CSF-PCR was performed for herpes simplex virus, varicella zoster virus, cytomegalovirus (CMV), JC virus (JCV), Epstein-Barr virus (EBV), Toxoplasma gondii and Mycobacterium tuberculosis. A definitive diagnosis was obtained by brain biopsy in 14 patients (2 with astrocytoma, 12 with brain infection). CSF-PCR was positive for EBV DNA in 3 of 3 cases of primary cerebral lymphoma, positive for JCV DNA in 6 of 7 biopsy-proven (and one autopsy-proven) cases of progressive multifocal leukoencephalopathy (PML). CSF-PCR was positive for CMV DNA in one biopsy-proven and one autopsy-proven case of CMV encephalitis (the former also had PML) and positive for M. tuberculosis DNA in one case of tuberculous encephalitis. None of the five toxoplasmic encephalitis cases (one definite, four presumptive) were T. gondii DNA positive. There was close correlation between histology and CSF-PCR for CMV encephalitis, PML and PCL. Antitoxoplasma therapy affected the sensitivity of both histological and CSF-PCR methods.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2024-03-19
    Description: A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Prokaryotes were quantified by flow citometry (CytoFLEXflex S, Beckman Coulter) as previously described by Gasol et al. (1999) with our own adjustment of the scatter settings to more accurately resolve these cells with the new CytoFLEX flow cytometry technology. Measurements were performed every month from different parts of the aquaria setup: the input seawater, the reservoir tank (after the filtration of seawater to 5 µm and before the UV lamp) and every experimental aquaria.
    Keywords: Atlantic Ocean; Comment; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; Flow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R); flow cytometry; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Prokaryotes; prokaryotic abundance; Replicate; Species; Species, unique identification (Semantic URI); Species, unique identification (URI); Type of study
    Type: Dataset
    Format: text/tab-separated-values, 1260 data points
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2024-03-19
    Description: A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Raspberry-based controllers were set to modify and monitor the water temperature, pH and dissolved oxygen in every treatment. Values for temperature (ºC), pH (NBS scale) and oxygen (% air saturation) were registered every 15 minutes in a database. The parameters were measured using PT100 temperature sensors, and Atlas Scientific Lab Grade pH and Dissolved Oxygen sensors. Every set of sensors was placed on each header tank, corresponding to each treatment (8 in total). Parameter setpoint values for each header tank were finely adjusted on the controller to ensure the treatment target values on each experimental aquaria. Oxygen sensors from treatments with ambient oxygen (~8.63 mg/L) were removed from the system due to calibration issues. Here, an example of the results for temperature, pH and oxygen for every header tank over 24 hours on 31st of December 2022 is presented. Values for pH (total scale) and dissolved oxygen (mg/L) were calculated using AquaEnv (Hofmann et al. 2010) and respR (Harianto et al. 2019) R packages, respectively, using the in situ temperature and salinity (35.1).
    Keywords: Atlantic Ocean; Calculated using AquaEnv R package; Calculated using respR R package; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Oxygen, dissolved; Oxygen saturation; Oxygen sensor, Atlas Scientific Lab grade; pH; pH sensor, Atlas Scientific, lab grade; Temperature, water; Thermometer, PT100; Type of study
    Type: Dataset
    Format: text/tab-separated-values, 3984 data points
    Location Call Number Limitation Availability
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