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  • 551  (1)
  • ABYSS; Arctic Ocean; ARK-XXVIII/2; Assessment of bacterial life and matter cycling in deep-sea surface sediments; Date/Time of event; deep sea sediment; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Dissolved Organic Matter; EGI; Elevation of event; Event label; Fourier-transform ion cyclotron mass spectrometry; Fram Strait; HAUSGARTEN 2013; HG_I; HG_II; HGI; HGIV; HGIX; HGVI; KH; Latitude of event; Light frame on-sight keyspecies investigation; LOKI; Longitude of event; Maria S. Merian; Mass spectrometer Solarix FT-ICR; Molecular mass; MSM29; MSM29_424-3; MSM29_425-3; MSM29_427-2; MSM29_432-2; MSM29_439-3; MSM29_443-2; MSM29_450-2; MUC; MultiCorer; Multicorer with television; N_4; North Greenland Sea; Number of molecular formulas; Percentage of total formulae; Polarstern; porewater; PS85; PS85/436-1; PS85/441-1; PS85/445-1; PS85/454-3; PS85/454-4; PS85/454-5; PS85/460-4; PS85/463-1; PS85/464-1; PS85/465-4; PS85/468-1; PS85/469-2; PS85/470-3; PS85/473-3; S_1; S_2; S_3; Sample ID; Station label; TVMUC  (1)
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  • 1
    Publication Date: 2024-02-02
    Description: Molecular analysis of dissolved organic matter in overlying bottom water and porewater was performed on a Solarix FT-ICR-MS equipped with a 15 Tesla superconducting magnet (Bruker Daltonic) using an electrospray ionization source (Bruker Apollo II) in negative ion mode. Molecular formula calculation for all samples was performed using a Matlab (2010) routine that searches, with an error of 〈 0.5 ppm, for all potential combinations of elements including the elements C∞, O∞, H∞, N ≤ 4; S ≤ 2 and P ≤ 1. Combination of elements NSP, N2S, N3S, N4S, N2P, N3P, N4P, NS2, N2S2, N3S2, N4S2, S2P was not allowed. Mass peak intensities are normalized relative to the total molecular formulas in each sample according to previously published rules (Rossel et al., 2015; doi:10.1016/j.marchem.2015.07.002). Additionally, the contribution of molecular formulas with different elemental composition (carbon = C; hydrogen = H; oxygen = O; nitrogen = N; sulfur = S; phosphorus = P) is expressed as a percentage of the total intensity in each sample. Furthermore, in this table,Molecular weight~wa~ was calculated considering the intensity of the mass peak in each sample (i.e. wa stands for intensity weighted-average).
    Keywords: ABYSS; Arctic Ocean; ARK-XXVIII/2; Assessment of bacterial life and matter cycling in deep-sea surface sediments; Date/Time of event; deep sea sediment; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Dissolved Organic Matter; EGI; Elevation of event; Event label; Fourier-transform ion cyclotron mass spectrometry; Fram Strait; HAUSGARTEN 2013; HG_I; HG_II; HGI; HGIV; HGIX; HGVI; KH; Latitude of event; Light frame on-sight keyspecies investigation; LOKI; Longitude of event; Maria S. Merian; Mass spectrometer Solarix FT-ICR; Molecular mass; MSM29; MSM29_424-3; MSM29_425-3; MSM29_427-2; MSM29_432-2; MSM29_439-3; MSM29_443-2; MSM29_450-2; MUC; MultiCorer; Multicorer with television; N_4; North Greenland Sea; Number of molecular formulas; Percentage of total formulae; Polarstern; porewater; PS85; PS85/436-1; PS85/441-1; PS85/445-1; PS85/454-3; PS85/454-4; PS85/454-5; PS85/460-4; PS85/463-1; PS85/464-1; PS85/465-4; PS85/468-1; PS85/469-2; PS85/470-3; PS85/473-3; S_1; S_2; S_3; Sample ID; Station label; TVMUC
    Type: Dataset
    Format: text/tab-separated-values, 957 data points
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2021-03-29
    Description: Due to its extreme salinity and high Mg concentration the Dead Sea is characterized by a very low density of cells most of which are Archaea. We discovered several underwater fresh to brackish water springs in the Dead Sea harboring dense microbial communities. We provide the first characterization of these communities, discuss their possible origin, hydrochemical environment, energetic resources and the putative biogeochemical pathways they are mediating. Pyrosequencing of the 16S rRNA gene and community fingerprinting methods showed that the spring community originates from the Dead Sea sediments and not from the aquifer. Furthermore, it suggested that there is a dense Archaeal community in the shoreline pore water of the lake. Sequences of bacterial sulfate reducers, nitrifiers iron oxidizers and iron reducers were identified as well. Analysis of white and green biofilms suggested that sulfide oxidation through chemolitotrophy and phototrophy is highly significant. Hyperspectral analysis showed a tight association between abundant green sulfur bacteria and cyanobacteria in the green biofilms. Together, our findings show that the Dead Sea floor harbors diverse microbial communities, part of which is not known from other hypersaline environments. Analysis of the water’s chemistry shows evidence of microbial activity along the path and suggests that the springs supply nitrogen, phosphorus and organic matter to the microbial communities in the Dead Sea. The underwater springs are a newly recognized water source for the Dead Sea. Their input of microorganisms and nutrients needs to be considered in the assessment of possible impact of dilution events of the lake surface waters, such as those that will occur in the future due to the intended establishment of the Red Sea - Dead Sea water conduit.
    Keywords: Dead Sea; Archaeal community ; 551
    Language: English
    Type: article , publishedVersion
    Format: 21
    Location Call Number Limitation Availability
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