In:
Molecular Nutrition & Food Research, Wiley, Vol. 54, No. 9 ( 2010-09), p. 1329-1339
Abstract:
Glycyrrhiza uralensis (licorice) is one of the most frequently prescribed ingredients in Oriental medicine, and licorice extract has been shown to exert anti‐carcinogenic effects. However, its use as a cancer chemopreventive agent is rather limited, due to the fact that its principal component, glycyrrhizin, is known to induce hypertension. This study determined the effects of a hexane/ethanol extract of G. uralensis (HEGU), which contains undetectable amounts of glycyrrhizin, on the apoptosis of androgen‐insensitive DU145 cells. HEGU induced apoptosis and increased the levels of cleaved caspase‐9, caspase‐7, caspase‐3 and poly (ADP‐ribose) polymerase (PARP). HEGU also induced mitochondrial membrane depolarization and cytochrome c release to the cytosol. HEGU increased the levels of Fas, death receptor 4 (DR4), cleaved caspase‐8, Mcl‐1S, and truncated Bid proteins. A caspase‐8 inhibitor suppressed HEGU‐induced apoptosis. An active fraction of HEGU was separated via column chromatography and the structure of the active compound isoangustone A was identified via 1 H‐NMR and 13 C‐NMR. Isoangustone A increased apoptotic cells, the cleavage of PARP and caspases, and the levels of DR4 and Mcl‐1S. Transfection with DR4 small interfering RNA attenuated HEGU‐ and isoangustone A‐induced apoptosis. These results demonstrate that the activation of DR4 contributes to HEGU‐ and isoangustone A‐induced apoptosis of DU145 cells.
Type of Medium:
Online Resource
ISSN:
1613-4125
,
1613-4133
DOI:
10.1002/mnfr.200900260
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
2160372-8
SSG:
12
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