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  • 1
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    A Pilot Study to Investigate the Efficacy of Fibrin Sealant (Tisseel®) in the Loop Electrosurgical Excision Procedure
    S. Karger AG ; 2015
    In:  Gynecologic and Obstetric Investigation Vol. 80, No. 1 ( 2015), p. 21-25
    Details
    In: Gynecologic and Obstetric Investigation, S. Karger AG, Vol. 80, No. 1 ( 2015), p. 21-25
    Abstract: 〈 b 〉 〈 i 〉 Aims: 〈 /i 〉 〈 /b 〉 The objective of the current study was to evaluate the efficacy and feasibility of fibrin sealant (Tisseel®) in the loop electrosurgical excision procedure (LEEP) for cervical intraepithelial neoplasia (CIN 2 or 3). 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 We designed a single-blind, prospective, randomized study in 40 consecutive women undergoing LEEP for CIN 2 or 3 at our institute. Two milliliters of fibrin sealant (Tisseel) was applied to the uterine cervix of 20 women immediately after LEEP surgery (treatment group). We evaluated abdominal pain, vaginal bleeding, vaginal discharge and impairment in daily living after 1 week using visual analogue scale questionnaires and compared the results with those of 20 women who did not receive fibrin sealant (control group). 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Among 40 women who returned for a follow-up 1 week after LEEP, 25 women (62.5%) reported at least one moderate to severe postprocedural symptom. The mean duration of moderate to severe vaginal bleeding and impairment in daily living during postoperative week 1 for the treatment group and the control group was 0.3 ± 0.80 versus 1.7 ± 2.36 days (p = 0.015) and 0.9 ± 1.37 versus 3.00 ± 2.62 days (p = 0.060), respectively. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 Intraoperative application of fibrin sealant (Tisseel) in LEEP can decrease postoperative vaginal bleeding and impairment in daily living.
    Type of Medium: Online Resource
    ISSN: 0378-7346 , 1423-002X
    URL: Article
    DOI: 10.1159/000369391
    RVK:
    XA 45603
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2015
    detail.hit.zdb_id: 1482695-1
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  • 2
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    Selection of Elderly Acute Myeloid Leukemia Patients for Intensive Chemotherapy: Effectiveness of Intensive Chemotherapy and Subgroup Analysis
    S. Karger AG ; 2015
    In:  Acta Haematologica Vol. 133, No. 3 ( 2015), p. 300-309
    Details
    In: Acta Haematologica, S. Karger AG, Vol. 133, No. 3 ( 2015), p. 300-309
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Despite the advances in acute myeloid leukemia (AML) treatment, the prognosis of elderly patients remains poor and no definitive treatment guideline has been established. In the present study, we aimed to evaluate the effectiveness of intensive chemotherapy in elderly AML patients and to determine which subgroup of patients would be most responsive to the therapy. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 We retrospectively analyzed 84 elderly patients: 35, 19, and 30 patients were administered intensive chemotherapy, low-dose chemotherapy, and supportive care, respectively. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Among those who received intensive chemotherapy, there were 17 cases of remission after induction chemotherapy; treatment-related mortality was 22.9%. The median overall survival was 7.9 months. Multivariate analysis indicated that the significant prognostic factors for overall survival were performance status, fever before treatment, platelet count, blast count, cytogenetic risk category, and intensive chemotherapy. Subgroup analysis showed that intensive chemotherapy was markedly effective in the relatively younger patients (65-70 years) and those with de novo AML, better-to-intermediate cytogenetic risk, no fever before treatment, high albumin levels, and high lactate dehydrogenase levels. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 Elderly AML patients had better outcomes with intensive chemotherapy than with low-intensity chemotherapy. Thus, appropriate subgroup selection for intensive chemotherapy is likely to improve therapeutic outcome. © 2014 S. Karger AG, Basel
    Type of Medium: Online Resource
    ISSN: 0001-5792 , 1421-9662
    URL: Article
    DOI: 10.1159/000362777
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2015
    detail.hit.zdb_id: 1481888-7
    detail.hit.zdb_id: 80008-9
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  • 3
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    Cytomorphological Factors and BRAF Mutation Predicting Risk of Lymph Node Metastasis in Preoperative Liquid-Based Fine Needle Aspirations of Papillary Thyroid Carcinoma
    S. Karger AG ; 2013
    In:  Acta Cytologica Vol. 57, No. 3 ( 2013), p. 252-258
    Details
    In: Acta Cytologica, S. Karger AG, Vol. 57, No. 3 ( 2013), p. 252-258
    Abstract: 〈 b 〉 〈 i 〉 Objective: 〈 /i 〉 〈 /b 〉 Preoperative fine needle aspiration biopsy (FNAB) has become the initial diagnostic method for papillary thyroid carcinoma (PTC). Identification of cytomorphologic factors predicting lymph node metastasis (LNM) is clinically important for determining the appropriate treatment regimen due to the high rate of lymph node involvement in PTC at the time of diagnosis. Hobnail features (HF) have previously been described as potential histomorphologic features in the histological examination of PTC. This study evaluated the value of HF as a predictor of LNM. 〈 b 〉 〈 i 〉 Study Design: 〈 /i 〉 〈 /b 〉 Histologically confirmed FNABs (n = 111) of papillary thyroid microcarcinoma prepared by the liquid-based method were enrolled. Along with other cytomorphologic parameters, HF were evaluated for their value in predicting LNM. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Although HF were closely correlated with cytoplasmic vacuoles of tumor cells and background macrophages (p 〈 0.05), which were considered diagnostic indicators of PTC with cystic changes, HF were only found to be significantly correlated with LNM (p = 0.008). The BRAF 〈 sup 〉 V660E 〈 /sup 〉 mutation was not associated with LNM. All combinations including HF were revealed as stronger predictors of LNM (odds ratios: 2.254-2.524, p 〈 0.05). 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 HF, a distinctive cytomorphologic feature, may be used as a factor predicting LNM in preoperative FNAB of PTC.
    Type of Medium: Online Resource
    ISSN: 0001-5547 , 1938-2650
    URL: Article
    DOI: 10.1159/000343617
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2013
    detail.hit.zdb_id: 2256676-4
    SSG: 12
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  • 4
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    Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity
    S. Karger AG ; 2007
    In:  International Archives of Allergy and Immunology Vol. 144, No. 4 ( 2007), p. 267-274
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 144, No. 4 ( 2007), p. 267-274
    Abstract: 〈 i 〉 Background: 〈 /i 〉 The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. 〈 i 〉 Methods: 〈 /i 〉 cDNA of the 19-kD BW allergen was cloned and expressed in 〈 i 〉 Escherichia coli 〈 /i 〉 . Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. 〈 i 〉 Results: 〈 /i 〉 The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew ( 〈 i 〉 Ana 〈 /i 〉 o 1), English walnut ( 〈 i 〉 Jug r 〈 /i 〉 2) and 7 S globulin from 〈 i 〉 Sesamum indicum. 〈 /i 〉 The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839–1.012), p 〈 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725–0.995), p 〈 0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661–0.945), p = 0.002] . 〈 i 〉 Conclusions: 〈 /i 〉 The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000106315
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2007
    detail.hit.zdb_id: 1482722-0
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  • 5
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    Detection and Quantification of Pharaoh Ant Antigens in Household Dust Samples as Newly Identified Aeroallergens
    S. Karger AG ; 2007
    In:  International Archives of Allergy and Immunology Vol. 144, No. 3 ( 2007), p. 247-253
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 144, No. 3 ( 2007), p. 247-253
    Abstract: 〈 i 〉 Background: 〈 /i 〉 The widespread house ant, 〈 i 〉 Monomorium pharaonis 〈 /i 〉 (pharaoh ant, PA), was recently identified as a potential cause of respiratory allergies. However, there are no reports of the distribution of PA allergens in various environments. We developed specific ELISA inhibition assays and measured the distribution and amount of PA antigens in household dust samples. 〈 i 〉 Methods: 〈 /i 〉 Floor dust was collected at 3-month intervals from 56 homes in Seoul over a 1-year period. PA antigens in fine dusts were quantified by ELISA inhibition assays using rabbit anti-PA sera, and specific IgE to PA antigens in residents’ serum was measured by ELISA. 〈 i 〉 Results: 〈 /i 〉 In 18 of the 56 homes (32.1%), PA antigen was detected in at least 1 floor dust sample either from the living room or the kitchen. Levels of PA antigens showed seasonal variations with peaks in autumn and winter. The detection rate of PA antigens was significantly higher in homes with visual evidence of PA infestations (70%) than in homes without such infestations (23.9%; p 〈 0.05). However, a significant amount of PA antigens was still detected in uninfested homes. Thirteen of 113 (11.5%) residents were positive for PA-specific IgE. PA-specific IgE was detected more frequently in residents living in PA antigen-positive homes (19.6%) than in antigen-negative homes (4.8%; p 〈 0.05). 〈 i 〉 Conclusion: 〈 /i 〉 A considerable level of PA antigens is distributed in the indoor environment. Therefore, inhalant exposure to PA antigens can occur during domestic activities. These results suggest that PAs might be a significant source of aeroallergens in households.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000103999
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2007
    detail.hit.zdb_id: 1482722-0
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  • 6
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    Impact of Fetal Echocardiography on Trends in Disease Patterns and Outcomes of Congenital Heart Disease in a Neonatal Intensive Care Unit
    S. Karger AG ; 2010
    In:  Neonatology Vol. 98, No. 1 ( 2010), p. 41-46
    Details
    In: Neonatology, S. Karger AG, Vol. 98, No. 1 ( 2010), p. 41-46
    Abstract: 〈 i 〉 Background: 〈 /i 〉 Congenital heart disease (CHD) is the most common developmental malformation and the leading cause of neonatal mortality and morbidity. The introduction of fetal echocardiography has made prenatal diagnosis of CHD possible. 〈 i 〉 Objective: 〈 /i 〉 This study was conducted to investigate the impact of fetal echocardiography on the changing disease patterns and outcomes of CHD. 〈 i 〉 Methods: 〈 /i 〉 A retrospective analysis of data from infants with CHD admitted to the neonatal intensive care unit (NICU) of the Asan Medical Center during the time periods was performed. Period I (1994–1996) was considered representative of a period before the introduction of fetal echocardiography, while period II (2004–2006) represented a period of more extensive application of fetal echocardiography. 〈 i 〉 Results: 〈 /i 〉 A total of 164 patients were admitted to the NICU during period I and 320 during period II. The number of infants prenatally diagnosed with CHD was 5 of 164 (3.0%) in period I and 219 of 320 (68.4%) in period II (p 〈 0.05). The overall accuracy of fetal diagnosis was approximately 92%. Of the 3 CHD categories, there was a greater proportion of infants with ‘significant’ heart disease in period II than I (47 vs. 32%; p 〈 0.05). In contrast, there was a smaller proportion of infants with ‘simple’ heart defects in period II than I (22 vs. 40%; p 〈 0.05). The proportion of infants with ‘complex’ heart disease was similar in both periods (28% in period I and 31% in period II). The 1-year survival rate of patients with CHD has improved remarkably with time (70.1% in period I to 88.8% in period II). Multivariate analysis showed prenatal diagnosis and planned delivery in a tertiary NICU are factors affecting CHD outcomes, especially when defects are ‘complex’ (p 〈 0.01). 〈 i 〉 Conclusion: 〈 /i 〉 Fetal echocardiography has resulted in an increased frequency of prenatal CHD diagnosis, has altered the disease patterns observed in the NICU, and has resulted in better 1-year outcomes.
    Type of Medium: Online Resource
    ISSN: 1661-7800 , 1661-7819
    URL: Article
    DOI: 10.1159/000264673
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2010
    detail.hit.zdb_id: 2403535-X
    SSG: 12
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  • 7
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    Antinuclear Antibodies in Patients with Prurigo Pigmentosa: A Linkage or a Coincidence?
    S. Karger AG ; 2009
    In:  Dermatology Vol. 218, No. 1 ( 2009), p. 90-91
    Details
    In: Dermatology, S. Karger AG, Vol. 218, No. 1 ( 2009), p. 90-91
    Type of Medium: Online Resource
    ISSN: 1421-9832 , 1018-8665
    URL: Article
    DOI: 10.1159/000161126
    RVK:
    XA 37700
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2009
    detail.hit.zdb_id: 1482189-8
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  • 8
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    PEP-1-GRX-1 Modulates Matrix Metalloproteinase-13 and Nitric Oxide Expression of Human Articular Chondrocytes
    S. Karger AG ; 2017
    In:  Cellular Physiology and Biochemistry Vol. 41, No. 1 ( 2017), p. 252-264
    Details
    In: Cellular Physiology and Biochemistry, S. Karger AG, Vol. 41, No. 1 ( 2017), p. 252-264
    Abstract: Background: The protein transduction domain (PTD) enables therapeutic proteins to directly penetrate the membranes of cells and tissues, and has been increasingly utilized. Glutaredoxin-1 (GRX-1) is an endogenous antioxidant enzyme involved in the cellular redox homeostasis system. In this study, we investigated whether PEP-1-GRX-1, a fusion protein of GRX-1 and PEP-1 peptide, a PTD, could suppress catabolic responses in primary human articular chondrocytes and a mouse carrageenan-induced paw edema model. Methods: Human articular chondrocytes were isolated enzymatically from articular cartilage and cultured in a monolayer. The transduction efficiency of PEP-1-GRX-1 into articular chondrocytes was measured by western blot and immunohistochemistry. The effects of PEP-1-GRX-1 on matrix metalloproteinases (MMPs) and catabolic factor expression in interleukin (IL)-1β- and lipopolysaccharide (LPS)-treated chondrocytes were analyzed by real-time quantitative reverse transcription-polymerase chain reaction and western blot. The effect of PEP-1-GRX1 on the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light chain-enhancer of activated B cells (NF-κB) signaling pathway were also analyzed by western blot. Finally, the inhibitory effect of PEP-1-GRX-1 on MMP-13 production was measured in vivo in a mouse carrageenan-induced paw edema model. Results: PEP-1-GRX-1 significantly penetrated into human chondrocytes and mouse cartilage, whereas GRX-1 did not. PEP-1-GRX-1 significantly suppressed MMP-13 expression and nitric oxide (NO) production in LPS-stimulated chondrocytes, and NO production in IL-1β-stimulated chondrocytes, compared with GRX-1. In addition, PEP-1-GRX-1 decreased IL-1β- and LPS-induced activation of MAPK and NF-κB. In the mouse model of carrageenan-induced paw edema, PEP-1-GRX-1 significantly suppressed carrageenan-induced MMP-13 production as well as paw edema. Conclusion: These results demonstrate that PEP-1-GRX-1 can be transduced efficiently in vitro and in vivo into human chondrocytes and mouse cartilage tissue and downregulate catabolic responses in chondrocytes by inhibiting the MAPK and NF-κB pathway. PEP-1-GRX-1 thus has the potential to reduce catabolic responses in chondrocytes and cartilage.
    Type of Medium: Online Resource
    ISSN: 1015-8987 , 1421-9778
    URL: Article
    DOI: 10.1159/000456090
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2017
    detail.hit.zdb_id: 1482056-0
    SSG: 12
    SSG: 15,3
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  • 9
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    Incidental Cholecystectomy in Patients with Asymptomatic Gallstones Undergoing Surgery for Colorectal Cancer
    S. Karger AG ; 2015
    In:  Digestive Surgery Vol. 32, No. 3 ( 2015), p. 183-189
    Details
    In: Digestive Surgery, S. Karger AG, Vol. 32, No. 3 ( 2015), p. 183-189
    Abstract: 〈 b 〉 〈 i 〉 Background and Aims: 〈 /i 〉 〈 /b 〉 The feasibility of incidental cholecystectomy during colorectal cancer (CRC) surgery has not been determined as yet. We aimed to investigate the feasibility of incidental cholecystectomy during CRC surgery. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 The clinicopathologic data of patients who underwent CRC surgery between January 2004 and May 2011 were assessed. Patients with asymptomatic cholelithiasis were divided into groups that did and did not undergo incidental cholecystectomy. Their in-hospital morbidity and long-term biliary complications were compared. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Of the 282 patients identified, 143 (50.7%) underwent incidental cholecystectomy and 139 (49.3%) were observed without cholecystectomy. The two groups were similar in clinical characteristics, except for gender and operation time. Only one patient (0.7%) in the cholecystectomy group experienced an intraoperative biliary complication. There was no significant difference in overall in-hospital morbidity between the two groups. After a median follow-up period of 33 months, long-term biliary complications developed in 12 patients (8.6%) in the observation group, with 9 undergoing cholecystectomy. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 Incidental cholecystectomy was not associated with increased postoperative morbidity, whereas previously asymptomatic patients were at substantial long-term risk of becoming symptomatic. Thus, in the absence of clear contraindications, concomitant cholecystectomy might be a desirable treatment option during CRC surgery in patients with asymptomatic gallstones.
    Type of Medium: Online Resource
    ISSN: 0253-4886 , 1421-9883
    URL: Article
    DOI: 10.1159/000380961
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2015
    detail.hit.zdb_id: 1468560-7
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  • 10
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    Characteristics of Perfusion Computed Tomography Imaging in Patients with Seizures Mimicking Acute Stroke
    S. Karger AG ; 2020
    In:  European Neurology Vol. 83, No. 1 ( 2020), p. 56-64
    Details
    In: European Neurology, S. Karger AG, Vol. 83, No. 1 ( 2020), p. 56-64
    Abstract: 〈 b 〉 〈 i 〉 Introduction: 〈 /i 〉 〈 /b 〉 Seizures as acute stroke mimics are a diagnostic challenge. 〈 b 〉 〈 i 〉 Objective: 〈 /i 〉 〈 /b 〉 The aim of the study was to characterize the perfusion patterns on perfusion computed tomography (PCT) in patients with seizures masquerading as acute stroke. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 We conducted a study on patients with acute seizures as stroke mimics. The inclusion criteria for this study were patients (1) initially presenting with stroke-like symptoms but finally diagnosed to have seizures and (2) with PCT performed within 72 h of seizures. The PCT of seizure patients ( 〈 i 〉 n 〈 /i 〉 = 27) was compared with that of revascularized stroke patients ( 〈 i 〉 n 〈 /i 〉 = 20) as the control group. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Among the 27 patients with seizures as stroke mimics, 70.4% ( 〈 i 〉 n 〈 /i 〉 = 19) showed characteristic PCT findings compared with the revascularized stroke patients, which were as follows: (1) multi-territorial cortical hyperperfusion {(73.7% [14/19] vs. 0% [0/20] , 〈 i 〉 p 〈 /i 〉 = 0.002), sensitivity of 73.7%, negative predictive value (NPV) of 80%}, (2) involvement of the ipsilateral thalamus {(57.9% [11/19] vs. 0% [0/20] , 〈 i 〉 p 〈 /i 〉 = 0.007), sensitivity of 57.9%, NPV of 71.4%}, and (3) reduced perfusion time {(84.2% [16/19] vs. 0% [0/20] , 〈 i 〉 p 〈 /i 〉 = 0.001), sensitivity of 84.2%, NPV of 87%}. These 3 findings had 100% specificity and positive predictive value in predicting patients with acute seizures in comparison with reperfused stroke patients. Older age was strongly associated with abnormal perfusion changes ( 〈 i 〉 p 〈 /i 〉 = 0.038), with a mean age of 66.8 ± 14.5 years versus 49.2 ± 27.4 years (in seizure patients with normal perfusion scan). 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 PCT is a reliable tool to differentiate acute seizures from acute stroke in the emergency setting.
    Type of Medium: Online Resource
    ISSN: 0014-3022 , 1421-9913
    URL: Article
    DOI: 10.1159/000506591
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2020
    detail.hit.zdb_id: 1482237-4
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