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  • S. Karger AG  (19)
  • Medicine  (19)
  • 1
    Online Resource
    Online Resource
    Cytomegalovirus Infection Is a Risk Factor in Gastrointestinal Cancer: A Cross-Sectional and Meta-Analysis Study
    S. Karger AG ; 2020
    In:  Intervirology Vol. 63, No. 1-6 ( 2020), p. 10-16
    Details
    In: Intervirology, S. Karger AG, Vol. 63, No. 1-6 ( 2020), p. 10-16
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 This study was planned to investigate the association between 〈 b 〉 〈 i 〉 〈 /i 〉 〈 /b 〉 human cytomegalovirus (HCMV) infection and gastrointestinal cancer (GIC) risk, by undertaking a meta-analysis and case-control cross-sectional study. 〈 b 〉 〈 i 〉 Summary: 〈 /i 〉 〈 /b 〉 A cross-sectional study analysis of 160 GIC patients and 100 control subjects indicated significantly higher HCMV prevalence in GIC patients based on the HCMV IgM test. However, a similar analysis based on an IgG test revealed no significant relationship. Further meta-analysis of 11 studies, including 1,044 patients and 991 healthy subjects, displayed HCMV infection as an important risk factor for not only colorectal cancer occurrence and development based on a HCMV DNA test, but also for GIC based on a HCMV IgM test. However, the IgG test again displayed no significant relationship between HCMV infection and GIC occurrence. 〈 b 〉 〈 i 〉 Key Message: 〈 /i 〉 〈 /b 〉 Overall, our study revealed that HCMV infection is associated with an increased GIC risk. However, additional studies are warranted to elucidate the molecular mechanism underlying this association.
    Type of Medium: Online Resource
    ISSN: 0300-5526 , 1423-0100
    URL: Article
    DOI: 10.1159/000506683
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2020
    detail.hit.zdb_id: 1482863-7
    SSG: 12
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  • 2
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    Transcatheter Aortic Valve Replacement for Pure Native Aortic Valve Regurgitation: A Systematic Review
    S. Karger AG ; 2018
    In:  Cardiology Vol. 141, No. 3 ( 2018), p. 132-140
    Details
    In: Cardiology, S. Karger AG, Vol. 141, No. 3 ( 2018), p. 132-140
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Transcatheter aortic valve replacement (TAVR) is a recent and an effective treatment option for high- or extreme-surgical-risk patients with symptomatic severe aortic stenosis. However, pure severe native aortic valve regurgitation (NAVR) without aortic stenosis remains a contraindication to TAVR. The aim of our systemic review analysis was to evaluate TAVR in patients with pure NAVR. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 We searched the published articles in the PubMed and Web of Science databases (2002–2017) using the Boolean operators for studies of NAVR patients undergoing TAVR. Reference lists of all returned articles were searched recursively for other relevant citations. Pooled estimates were calculated using a random-effects meta-analysis. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Finally, a total of 10 studies were included in this analysis. The CoreValve was more frequently used with a lower rate of device success and a higher rate of residual aortic regurgitation. The new-generation transcatheter heart valves (THVs) performed a significantly higher rate with less residual aortic regurgitation and a success rate close to 100%. The 30-day all-cause mortality rates ranged from 0 to 30% with an estimate summary rate of 9% (95% CI: 5–15%; 〈 i 〉 I 〈 /i 〉 〈 sup 〉 2 〈 /sup 〉 = 33%). Cerebrovascular events, major or life-threatening bleeding, major vascular complications, acute kidney disease, and new permanent pacemaker implantation occurred similarly in both the new- and old-generation THV devices. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 Aortic regurgitation remains a challenging pathology for TAVR. TAVR is a feasible and reasonable option for carefully selected patients with pure aortic regurgitation.
    Type of Medium: Online Resource
    ISSN: 0008-6312 , 1421-9751
    URL: Article
    DOI: 10.1159/000491919
    RVK:
    XA 36200
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
    detail.hit.zdb_id: 1482041-9
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  • 3
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    Whole Genome Sequence Identified a Rare Homozygous Pathogenic Mutation of the DSG2 Gene in a Familial Arrhythmogenic Cardiomyopathy Involving Both Ventricles
    S. Karger AG ; 2017
    In:  Cardiology Vol. 138, No. 1 ( 2017), p. 41-54
    Details
    In: Cardiology, S. Karger AG, Vol. 138, No. 1 ( 2017), p. 41-54
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 This study was designed to identify the pathogenic mutation in a Chinese family with arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) using whole genome sequencing (WGS). 〈 b 〉 〈 i 〉 Methods and Results: 〈 /i 〉 〈 /b 〉 Probands II:1 and II:2 underwent routine examinations for diagnosis. Genomic DNA was extracted from the peripheral blood of family members and analyzed using WGS. A total of 60,285 single-nucleotide polymorphisms (SNP) and 13,918 insertions/deletions (InDel) occurring in the exonic regions of genes and predisposing to cardiomyopathies and arrhythmias were identified. When filtered using the 1000 Genomes Project (2014 version), NHLBI ESP6500, and ExAC databases, 12 missense SNP and 2 InDel in exonic regions remained, the allele frequencies of which were 〈 0.01 or unknown. The potentially pathogenic mutations that occurred in the genes DSG2, PKP4, PRKAG2, FOXD4, CTTN, and DMD, which were identified by SIFT or PolyPhen-2 software as “damaging,” were validated using Sanger sequencing. Probands II:1 and II:2 shared an extremely rare homozygous mutation in the DSG2 (p.F531C) gene, which was also demonstrated using intersection analysis of WGS data from probands II:1 and II:2. Electron microscopy and histological staining of myocardial biopsies showed widened and destroyed intercalated discs, and interrupted, atrophic, and disarranged myocardial fibers, and hyperplastic interstitial fibers, collagen fibers, and adipocytes were infiltrated and invaded. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 A homozygous mutation of DSG2 p.F531C was identified as the pathogenic mutation in patients with ARVC/D involving both ventricles, as a result of widened and impaired intercalated discs, interrupted myocardial fibers, and abnormally hyperplastic interstitial fibers, collagen fibers, and adipocytes.
    Type of Medium: Online Resource
    ISSN: 0008-6312 , 1421-9751
    URL: Article
    DOI: 10.1159/000462962
    RVK:
    XA 36200
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2017
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  • 4
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    Online Resource
    Partial Protein-Hydrolyzed Infant Formula Decreased Food Sensitization but Not Allergic Diseases in a Prospective Birth Cohort Study
    S. Karger AG ; 2011
    In:  International Archives of Allergy and Immunology Vol. 154, No. 4 ( 2011), p. 310-317
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 154, No. 4 ( 2011), p. 310-317
    Abstract: 〈 i 〉 Background: 〈 /i 〉 Exposure to cow’s milk protein in early infancy could lead to increased rates of allergic diseases later in life. We investigated whether feeding a protein-hydrolyzed formula (HF) in the first 6 months of life decreased allergic diseases up to 36 months later. 〈 i 〉 Methods: 〈 /i 〉 Newborns who had at least 1 first-degree family member with a history of atopy and could not breast-feed were enrolled. They were fed with HF or cow’s milk infant formula (CM) for at least 6 months via an open-label protocol and were monitored prospectively at 6, 18 and 36 months of age to assess allergy sensitization and allergic diseases. 〈 i 〉 Results: 〈 /i 〉 A total of 1,002 infants were enrolled and 679 infants were consistently fed the same formula for the first 6 months of life (345 HF and 334 CM). The percentage of food sensitization (especially to milk protein) was significantly lower in the HF group than in the CM group at 36 months (12.7 vs. 23.4%, p = 0.048). There was no significant difference in the prevalence of aeroallergen sensitization between the groups. Occurrence of allergic diseases during the first 3 years of life was significantly correlated with aeroallergen sensitization, but not to food allergen sensitization, parental atopy or feeding types. 〈 i 〉 Conclusions: 〈 /i 〉 Infants fed with HF during the first 6 months of life had a significantly lower percentage of sensitization to milk protein allergens, but not allergic diseases during the first 3 years of life. Avoidance of cow’s milk protein alone in infancy is not enough to decrease rates of allergic diseases.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000321823
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2011
    detail.hit.zdb_id: 1482722-0
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  • 5
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    Wernicke’s Encephalopathy in a Patient with Multiple Symmetrical Lipomatosis and the A8344G Mutation of Mitochondrial DNA
    S. Karger AG ; 2002
    In:  European Neurology Vol. 47, No. 2 ( 2002), p. 126-128
    Details
    In: European Neurology, S. Karger AG, Vol. 47, No. 2 ( 2002), p. 126-128
    Type of Medium: Online Resource
    ISSN: 0014-3022 , 1421-9913
    URL: Article
    DOI: 10.1159/000047967
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2002
    detail.hit.zdb_id: 1482237-4
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  • 6
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    Application of 16 G Biopsy Needle in Transperineal Template-Guided Prostate Biopsy
    S. Karger AG ; 2022
    In:  Urologia Internationalis Vol. 106, No. 9 ( 2022), p. 909-913
    Details
    In: Urologia Internationalis, S. Karger AG, Vol. 106, No. 9 ( 2022), p. 909-913
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 The aim of this study was to evaluate the clinical value of 16 G biopsy needle in transperineal template-guided prostate biopsy (TTPB), compared with 18 G biopsy needle. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 The patients who underwent TTPB from August 2020 to February 2021 were randomized into 2 groups using a random number table. The control group ( 〈 i 〉 n 〈 /i 〉 = 65) and the observation group ( 〈 i 〉 n 〈 /i 〉 = 58) performed biopsy with 18 G (Bard MC l820) and 16 G (Bard MC l616) biopsy needles, respectively. Positive rate of biopsy, Gleason score, complications, and pain score were statistically analyzed. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The age, prostate volume, PSA, and the number of cores were comparable between the 2 groups. The positive rate of biopsy in the observation group was 68.9% (40/58), meanwhile the control group was 46.2% (30/65). There was statistical difference between the 2 groups ( 〈 i 〉 p 〈 /i 〉 = 0.011). Gleason score of the observation group (8 [7–9]) was higher than that of the control group (8 [6–9] ) ( 〈 i 〉 p 〈 /i 〉 = 0.038). There was no significant difference in pain score and complications including hematuria, hematospermia, perineal hematoma, infection, and urinary retention between the 2 groups ( 〈 i 〉 p 〈 /i 〉 & #x3e; 0.05). 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 16 G biopsy needle significantly improved the positive rates and accurately evaluate the nature of lesions, meanwhile did not increase the incidence of complications compared with 18 G biopsy needle.
    Type of Medium: Online Resource
    ISSN: 0042-1138 , 1423-0399
    URL: Article
    DOI: 10.1159/000520373
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2022
    detail.hit.zdb_id: 1464417-4
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  • 7
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    Coronary Artery Calcium Determined by Electron Beam Computed Tomography for Predicting Angiographic Coronary Artery Disease in Moderate- to High-Risk Chinese Patients
    S. Karger AG ; 2001
    In:  Cardiology Vol. 95, No. 4 ( 2001), p. 183-189
    Details
    In: Cardiology, S. Karger AG, Vol. 95, No. 4 ( 2001), p. 183-189
    Abstract: The aim of this study was to evaluate the prevalence of coronary calcification among moderate- to high-risk Chinese patients and to evaluate the ability of the coronary calcium score determined by electron beam computed tomography (EBCT) to predict angiographic coronary artery disease in this population. We enrolled 163 consecutive patients and analyzed their cardiovascular risk factors, coronary calcium scores and coronary angiogram results. One hundred and twenty-five patients (76.7%) had a positive EBCT scan result (coronary calcium score 〉 0). The prevalence of calcification and the calcium scores showed a graded relation to the number of cardiovascular risk factors and age (p 〈 0.001 for trend). Coronary calcium scores showed statistically significant differences between patients with angiographic evidence of coronary artery disease and patients with normal coronary angiography (p 〈 0.05), but could not differentiate between patients with significant and insignificant coronary artery disease. Receiver operating characteristic curve analysis showed that a coronary calcium score 〉 5 predicted angiographic coronary artery disease with 93% sensitivity and 86% specificity (area under the curve 0.95 ± 0.019). Multivariate analysis showed a coronary calcium score 〉 5 to be the strongest independent predictor of angiographic coronary artery disease (odds ratio 120.7, 95% confidence interval 21.7–671.4; p 〈 0.001). Coronary calcium score determined by EBCT appears to have a similar predictive value in Chinese patients as it does in other ethnic populations that have been reported to date.
    Type of Medium: Online Resource
    ISSN: 0008-6312 , 1421-9751
    URL: Article
    DOI: 10.1159/000047370
    RVK:
    XA 36200
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2001
    detail.hit.zdb_id: 1482041-9
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  • 8
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    His6-Tagged UL35 Protein of Duck Plague Virus: Expression, Purification, and Production of Polyclonal Antibody
    S. Karger AG ; 2009
    In:  Intervirology Vol. 52, No. 3 ( 2009), p. 141-151
    Details
    In: Intervirology, S. Karger AG, Vol. 52, No. 3 ( 2009), p. 141-151
    Abstract: 〈 i 〉 Objective: 〈 /i 〉 Duck plague virus (DPV), the causative agent of duck plague (DP), is an alphaherpesvirus that causes an acute, febrile, contagious, and septic disease of waterfowl. UL35 protein (VP26) is a major capsid protein encoded by the UL35 gene, which is located in the unique long (UL) region of the DPV genome. To investigate the specific roles of VP26, the UL35 gene was amplified from the DPV DNA by polymerase chain reaction (PCR) and subcloned into pET-32a(+). 〈 i 〉 Methods: 〈 /i 〉 The resultant prokaryotic expression vector, pET-32a(+)/UL35, includes an amino-terminal His6 as a fusion partner. 〈 i 〉 Escherichia coli 〈 /i 〉 BL21 (DE3) competent cells were transformed with the construct and protein expression was subsequently induced by the addition of isopropyl-β- 〈 i 〉 D 〈 /i 〉 -thiogalactopyranoside to the culture medium. Protein lysates were submitted to SDS-PAGE to evaluate recombinant protein expression. 〈 i 〉 Results: 〈 /i 〉 The band that corresponded to the predicted protein size (33 kDa) was observed on the SDS-PAGE gel. The recombinant His6-tagged VP26 fusion protein was expressed at a high level in an insoluble form (inclusion bodies) and constituted about 24% of the total cellular protein. Then, the fusion protein was purified to near homogeneity using single-step immobilized metal affinity chromatography on a nickel-nitrilotriacetic acid affinity resin, yielding about 620 mg per liter culture. After purification, New Zealand white rabbits were immunized with purified His6-tagged VP26 in order to raise polyclonal antibody against this recombinant protein. Using the resultant sera, Western blot analysis showed that the recombinant protein was recognized by the polyclonal antibody. 〈 i 〉 Conclusion: 〈 /i 〉 Thus, the polyclonal antibody prepared here may serve as a valuable tool to study the functional involvement of VP26 in the DPV life cycle.
    Type of Medium: Online Resource
    ISSN: 0300-5526 , 1423-0100
    URL: Article
    DOI: 10.1159/000221833
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2009
    detail.hit.zdb_id: 1482863-7
    SSG: 12
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  • 9
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    Characterization of Synonymous Codon Usage Bias in the Duck Plague Virus UL35 Gene
    S. Karger AG ; 2009
    In:  Intervirology Vol. 52, No. 5 ( 2009), p. 266-278
    Details
    In: Intervirology, S. Karger AG, Vol. 52, No. 5 ( 2009), p. 266-278
    Abstract: 〈 i 〉 Objective: 〈 /i 〉 The aim was to identify the codon usage bias between the newly identified duck plague virus (DPV) UL35 gene (GenBank accession No. EF643558) and the UL35-like genes of 27 other reference herpesviruses. 〈 i 〉 Methods: 〈 /i 〉 A comparative analysis of the codon usage bias of the 28 herpesviruses was performed by using the CodonW 1.4 program and CUSP (create a codon usage table) program of EMBOSS (The European Molecular Biology Open Software Suite). 〈 i 〉 Results: 〈 /i 〉 The results showed obvious differences of the synonymous codon usage bias in the 28 herpesviruses indicated by the Codon Adaptation Index, effective number of codons (ENc), and the value of G + C content at the 3rd codon position. The codon usage pattern of the DPV UL35 gene was phylogenetically conserved and similar to that of the UL35-like genes of the avian α-herpesvirus, with a strong bias towards the codons with A and T at the 3rd codon position. A cluster analysis of codon usage pattern of the DPV UL35 gene with other reference herpesviruses demonstrated that the codon usage bias of the UL35 genes of the 28 herpesviruses had a very close relation with their gene function. The ENc-plot revealed that the genetic heterogeneity in the DPV UL35 gene and the 27 reference herpesviruses were constrained by G + C content, while gene length exerted relatively weaker influences. In addition, comparisons of the codon preferences in the UL35 gene of DPV with those of 〈 i 〉 Escherichia coli 〈 /i 〉 , yeast and humans revealed that there were 33 codons showing distinct usage differences between the DPV and yeast, and 38 between the DPV and humans, but only 31 between the DPV and 〈 i 〉 E. coli 〈 /i 〉 . Therefore, the 〈 i 〉 E. coli 〈 /i 〉 system may be more suitable for the expression of the DPV UL35 gene. 〈 i 〉 Conclusion: 〈 /i 〉 Together, these results may improve our understanding of the evolution, pathogenesis and functional studies of DPV, as well as contribute significantly to the area of herpesvirus research and possibly studies with other viruses.
    Type of Medium: Online Resource
    ISSN: 0300-5526 , 1423-0100
    URL: Article
    DOI: 10.1159/000231992
    RVK:
    XA 10000
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2009
    detail.hit.zdb_id: 1482863-7
    SSG: 12
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  • 10
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    Component-Resolved Analyses for Diagnosis of Food Allergies in Infants and Young Children in Southern China
    S. Karger AG ; 2023
    In:  International Archives of Allergy and Immunology Vol. 184, No. 7 ( 2023), p. 668-680
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 184, No. 7 ( 2023), p. 668-680
    Abstract: 〈 b 〉 〈 i 〉 Introduction: 〈 /i 〉 〈 /b 〉 Chicken eggs and cow’s milk are two of the most common foods that cause allergic reactions in infants and young children, and there is a lack of precise diagnostic methods to identify the allergic state of these patients. The recently developed food allergen component-resolved diagnosis (CRD) may be a more accurate diagnosis method for food allergies. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 One hundred children sensitized to egg white and milk crude extracts and diagnosed with or suspected allergic disease were included. The specific immunoglobulin E (sIgE) (s) of animal food allergen crude extracts (egg yolk, milk, shrimp, crab, cod, beef) and the main components of egg white and milk were tested. The sensitization characteristics, cross-reactivity, and clinical relevance were analyzed. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The results of egg white-sensitized patients showed that ovalbumin (Gal d 2) had the highest positive rate of 100%. Compared with other pairwise combinations of egg allergens 〈 b 〉 , 〈 /b 〉 the combination of egg white and Gal d 2 had higher diagnostic accuracy, with an AUC of 0.876 (95% CI: 0.801–0.951), a sensitivity of 88.9%, and a specificity of 75.9%. The positive rates of beta-lactoglobulin (Bos d 5) and alpha-lactoglobulin (Bos d 4) in the milk-sensitized children were comparable, 92% and 91%, respectively. The combination of crude milk extract and Bos d 4 had the highest diagnostic accuracy, with an AUC of 0.969 (95% CI: 0.938–0.999), a sensitivity of 100%, and a specificity of 82.7%. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 Among these subjects, our study found the main allergenic component of egg white was Gal d 2, and the main allergenic components of milk were Bos d 4 and Bos d 5. CRD may help identify egg/milk allergies and non-allergies.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000529305
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2023
    detail.hit.zdb_id: 1482722-0
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