In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 4 ( 2023-4-3), p. e1011251-
Abstract:
Magnaporthe oryzae causes rice blasts posing serious threats to food security worldwide. During infection, M . oryzae utilizes several transmembrane receptor proteins that sense cell surface cues to induce highly specialized infectious structures called appressoria. However, little is known about the mechanisms of intracellular receptor tracking and their function. Here, we described that disrupting the coat protein complex II (COPII) cargo protein MoErv14 severely affects appressorium formation and pathogenicity as the Δ Moerv14 mutant is defective not only in cAMP production but also in the phosphorylation of the mitogen-activated protein kinase (MAPK) MoPmk1. Studies also showed that either externally supplementing cAMP or maintaining MoPmk1 phosphorylation suppresses the observed defects in the Δ Moerv14 strain. Importantly, MoErv14 is found to regulate the transport of MoPth11, a membrane receptor functioning upstream of G-protein/cAMP signaling, and MoWish and MoSho1 function upstream of the Pmk1-MAPK pathway. In summary, our studies elucidate the mechanism by which the COPII protein MoErv14 plays an important function in regulating the transport of receptors involved in the appressorium formation and virulence of the blast fungus.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011251
DOI:
10.1371/journal.ppat.1011251.g001
DOI:
10.1371/journal.ppat.1011251.g002
DOI:
10.1371/journal.ppat.1011251.g003
DOI:
10.1371/journal.ppat.1011251.g004
DOI:
10.1371/journal.ppat.1011251.g005
DOI:
10.1371/journal.ppat.1011251.g006
DOI:
10.1371/journal.ppat.1011251.g007
DOI:
10.1371/journal.ppat.1011251.g008
DOI:
10.1371/journal.ppat.1011251.g009
DOI:
10.1371/journal.ppat.1011251.s001
DOI:
10.1371/journal.ppat.1011251.s002
DOI:
10.1371/journal.ppat.1011251.s003
DOI:
10.1371/journal.ppat.1011251.s004
DOI:
10.1371/journal.ppat.1011251.s005
DOI:
10.1371/journal.ppat.1011251.s006
DOI:
10.1371/journal.ppat.1011251.s007
DOI:
10.1371/journal.ppat.1011251.s008
DOI:
10.1371/journal.ppat.1011251.s009
DOI:
10.1371/journal.ppat.1011251.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2205412-1
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