In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2021-1-7), p. e1009033-
Abstract:
The p53 transcription factor plays a key role both in cancer and in the cell-intrinsic response to infections. The ORFEOME project hypothesized that novel p53-virus interactions reside in hitherto uncharacterized, unknown, or hypothetical open reading frames (orfs) of human viruses. Hence, 172 orfs of unknown function from the emerging viruses SARS-Coronavirus, MERS-Coronavirus, influenza, Ebola, Zika (ZIKV), Chikungunya and Kaposi Sarcoma-associated herpesvirus (KSHV) were de novo synthesized, validated and tested in a functional screen of p53 signaling. This screen revealed novel mechanisms of p53 virus interactions and two viral proteins KSHV orf10 and ZIKV NS2A binding to p53. Originally identified as the target of small DNA tumor viruses, these experiments reinforce the notion that all viruses, including RNA viruses, interfere with p53 functions. These results validate this resource for analogous systems biology approaches to identify functional properties of uncharacterized viral proteins, long non-coding RNAs and micro RNAs.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009033
DOI:
10.1371/journal.ppat.1009033.g001
DOI:
10.1371/journal.ppat.1009033.g002
DOI:
10.1371/journal.ppat.1009033.g003
DOI:
10.1371/journal.ppat.1009033.g004
DOI:
10.1371/journal.ppat.1009033.g005
DOI:
10.1371/journal.ppat.1009033.g006
DOI:
10.1371/journal.ppat.1009033.g007
DOI:
10.1371/journal.ppat.1009033.g008
DOI:
10.1371/journal.ppat.1009033.s001
DOI:
10.1371/journal.ppat.1009033.s002
DOI:
10.1371/journal.ppat.1009033.s003
DOI:
10.1371/journal.ppat.1009033.s004
DOI:
10.1371/journal.ppat.1009033.s005
DOI:
10.1371/journal.ppat.1009033.s006
DOI:
10.1371/journal.ppat.1009033.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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