In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 11 ( 2020-11-19), p. e0242614-
Abstract:
The purpose of this study was to evaluate matrix metalloproteinases (MMP) -2 and MMP-3 in serum, and keratinocyte-derived chemoattractant (KC), interleukin 8 (IL-8) and monocyte chemoattractant 1 (MCP-1) in synovial fluid (SF) as stifle osteoarthritis (OA) biomarkers in dogs. Dogs with naturally occurring cranial cruciate ligament (CrCL) rupture (OA group) and healthy controls were recruited. Stifles with CrCL deficiency were surgically stabilized. Serum, SF, and synovial biopsy samples were collected from the OA group preoperatively, whereas samples were collected once from control dogs. A blinded veterinary pathologist graded synovial biopsies. Serum and SF analyses were performed using xMAP technology. General linear regression was used for statistical comparisons of serum biomarkers, and mixed linear regression for SF biomarkers and temporal concentration changes. The overall discriminative ability was quantified using area under curve (AUC). Spearman’s correlation coefficient was used to assess correlations between synovial histology grades and the biomarkers. Samples from 62 dogs in the OA group and 50 controls were included. The MMP-2 and MMP-3 concentrations between the OA and control groups were not significantly different, and both with an AUC indicating a poor discriminative ability. All three SF biomarker concentrations were significantly different between the OA group and controls ( P 〈 0.05). The MCP-1 was the only biomarker showing an acceptable discriminative performance with an AUC of 0.91 (95% confidence interval: 0.83–0.98). The sum of the inflammatory infiltrate score was significantly correlated with all three SF biomarkers ( P 〈 0.01). Summed synovial stroma, and all scores combined were significantly correlated with IL-8 and MCP-1 concentrations ( P 〈 0.003), and the summed synoviocyte scores were significantly correlated with MCP-1 concentrations ( P 〈 0.001). Correlations between MCP-1 concentrations and synovial histopathologic grading and its discriminative ability suggest its potential as a synovitis biomarker in canine stifle OA associated with CrCL rupture.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0242614
DOI:
10.1371/journal.pone.0242614.g001
DOI:
10.1371/journal.pone.0242614.g002
DOI:
10.1371/journal.pone.0242614.g003
DOI:
10.1371/journal.pone.0242614.g004
DOI:
10.1371/journal.pone.0242614.t001
DOI:
10.1371/journal.pone.0242614.t002
DOI:
10.1371/journal.pone.0242614.t003
DOI:
10.1371/journal.pone.0242614.t004
DOI:
10.1371/journal.pone.0242614.t005
DOI:
10.1371/journal.pone.0242614.s001
DOI:
10.1371/journal.pone.0242614.s002
DOI:
10.1371/journal.pone.0242614.s003
DOI:
10.1371/journal.pone.0242614.s004
DOI:
10.1371/journal.pone.0242614.s005
DOI:
10.1371/journal.pone.0242614.r001
DOI:
10.1371/journal.pone.0242614.r002
DOI:
10.1371/journal.pone.0242614.r003
DOI:
10.1371/journal.pone.0242614.r004
DOI:
10.1371/journal.pone.0242614.r005
DOI:
10.1371/journal.pone.0242614.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2267670-3
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