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  • 1
    In: Fishes, MDPI AG, Vol. 6, No. 2 ( 2021-03-28), p. 11-
    Abstract: Optimal crop production in aquaponics is influenced by water pH and potassium concentrations. The addition of potassium hydroxide (KOH) into the recirculating aquaculture system (RAS) may benefit aquaponics by increasing the water pH for better biofilter activity and supplementing K for better plant growth and quality. We investigated the growth, feed conversion, body composition and welfare indicators of juvenile African catfish (Clarias gariepinus) treated with four concentrations of K (K0 = 2, K200 = 218, K400 = 418, and K600 = 671 mg L−1). While growth, feed conversion and final body composition were unaffected, the feeding time and individual resting significantly increased with increasing K+. The swimming activity and agonistic behavior were reduced significantly under increased concentrations of K+. Leftover feed and the highest number of skin lesions were observed under K600. We suggest that K+ concentrations between 200 and 400 mg L−1 can improve the welfare status of juvenile African catfish. This enables the application of KOH in RAS to supply alkalinity to achieve optimum nitrification at minimum water exchange and improve the nutritional profile of the process water with benefits for the welfare status of African catfish and aquaponics plant production and quality.
    Type of Medium: Online Resource
    ISSN: 2410-3888
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
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  • 2
    In: AgriEngineering, MDPI AG, Vol. 3, No. 1 ( 2021-02-26), p. 92-109
    Abstract: Basil (Ocimum basilicum) was cultivated in Rostock, Northern Germany, in a decoupled aquaponic system with African catfish (Clarias gariepinus) under intensive rearing conditions by using three hydroponic components, the dynamic root floating technique (DRF), the raft technique, and grow pipes. A 25% of the recommended feed input still allowed African catfish growth and provided adequate nitrogen and calcium levels in the process water. After 36 days, the plants were examined with respect to 16 different growth parameters. DRF performed significantly better than raft and/or grow pipes in 11 parameters. Total weight of basil was significantly higher in DRF (107.70 ± 34.03 g) compared with raft (82.02 ± 22.74 g) and grow pipes (77.86 ± 23.93 g). The economically important leaf biomass was significantly higher in wet and dry weight under DRF cultivation (45.36 ± 13.53 g; 4.96 ± 1.57 g) compared with raft (34.94 ± 9.44 g; 3.74 ± 1.04 g) and grow pipes (32.74 ± 9.84 g; 3.75 ± 1.22 g). Two main factors limited plant growth: an unbalanced nutrient concentration ratio and high water temperatures with an average of 28 °C (max 34.4 °C), which resulted in reduced root activity in raft and grow pipes. DRF was able to maintain root activity through the 5 cm air space between the shoots and the nutrient solution and thus produced significantly more biomass. This suggests DRF to be used for basil aquaponics under glass house conditions with high-temperature scenarios. Future studies are needed to optimize nutrient loads and examine systems with the plant roots exposed to air (Aeroponics).
    Type of Medium: Online Resource
    ISSN: 2624-7402
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
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  • 3
    In: Water, MDPI AG, Vol. 15, No. 2 ( 2023-01-11), p. 299-
    Abstract: Fingerlings (0.23 g) and juveniles (267.04 g) of African catfish (Clarias gariepinus) were reared for 32 days under experimental aquarium conditions and were exposed to either 0.75 mg/L or 3.0 mg/L diethylenetriaminepentaacetic acid-iron(II) (Fe-DTPA) and 3.0 mg/L or 12.0 mg/L Fe-DTPA in the water, respectively. These treatment groups were compared to a control group without additional Fe-DTPA. The growth, mortality, ethological indicators (activity, agonistic interactions, air-breathing), leukocyte distribution, histopathological changes in liver and gills, and genetic biomarkers were evaluated for each group. While the growth, mortality, and behavior were not significantly different between the groups, the lymphocyte count in the fish’s blood increased significantly in all groups during the course of the experiment, but independently from the treatments. A similar trend (p 〉 0.05) was observed in monocytes. The number of granulocytes decreased significantly, but independently from the treatments. These changes indicated the possibility of an ongoing immune response in the fish from all treatments that might be caused by the increasing aggressive behavior of the fish. However, the Fe-DTPA treatments did not cause a notable suppression or enhancement of the immune reactions. Fe3+ accumulations in liver tissues were detected at the tested concentrations, and further changes occurred in the cells of the gills. Gene-expression biochips were used to simultaneously quantify the transcript levels of 34 genes associated with iron metabolism and stress physiology in head kidney samples. The obtained gene-expression profiles did not reveal any significant differences across either the different treatments or the time points. The results indicate that Fe-DTPA supplementation in the tested concentrations can be considered relatively harmless for the health and welfare of African catfish.
    Type of Medium: Online Resource
    ISSN: 2073-4441
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
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  • 4
    In: Sustainability, MDPI AG, Vol. 12, No. 20 ( 2020-10-21), p. 8717-
    Abstract: Aquaponics production of spearmint (Mentha spicata) was evaluated under commercial grow-out conditions of African catfish (Clarias gariepinus) in Northern Germany (Mecklenburg-Western Pomerania). Fish batch production under different stocking densities in an extensive aquacultural unit (EAU) and an intensive aquacultural unit (IAU) was connected to conventional plant cultivation on ebb-and-flood planting tables and compared to a liquid fertilizer control. The best growth parameters of M. spicata were found under the intensive stocking density of C. gariepinus (IAU), resulting in a plant leaf area of 10.9 ± 2.5 cm2, leaf length of 8.6 ± 1.6 cm, and a cut fresh biomass from aboveground of 31.8 ± 13.8 g/plant, compared to the EAU (5.6 ± 2.1 cm2; 5.4 ± 1.4 cm; 17.4 ± 4.7 g/plant) and the control (5.7 ± 2.2 cm2; 5.5 ± 1.4 cm; 11.2 ± 5.3 g/plant). The fresh biomass of the whole plants was not significantly different between the EAU (165.5 ± 71.7 g/plant) and the IAU (190.7 ± 105.6 g/plant), though the latter gained more weight. The initial fish number ratio between the EAU and the IAU of 1/4 increased the M. spicata leaf area by twofold in the IAU. Our results demonstrate that aquaponics (s.l.) production of M. spicata is possible under the direct use of effluent waters from intensive African catfish cultivation without the addition of any liquid fertilizer.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
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  • 5
    In: Sustainability, MDPI AG, Vol. 14, No. 11 ( 2022-05-27), p. 6607-
    Abstract: A new cultivation system with the chlorophyte Monoraphidium contortum combined with a self-sustaining culture of the freshwater rotifer Brachionus calyciflorus was applied for Sander lucioperca (L.) larviculture. Survival, morphometrics, as well as fatty acid composition of pikeperch larvae were analyzed after a ten-day feeding period. By using the pseudo-green water technique with improved aeration and water movement at the surface, survival rates reached up to 94%, with a total larval length of 8.1 ± 0.3 mm and a specific length growth rate of up to 4.1% day−1 for S. lucioperca. The biochemical composition of B. calyciflorus and especially its contents in C18 PUFAs and suitable n-3/n-6 ratios met the nutritional requirements of pikeperch larvae. The high abundance of highly unsaturated fatty acids (HUFAs) in the diet appeared to be less important in the first feeding due to a possible retention of essential fatty acids, which originate from the yolk sac reserves, at adequate levels. Exponential growth of microalgae and zooplankton under the applied conditions was most effective when stocking M. contortum five days and B. calyciflorus three days before adding the fish larvae. Appropriate timing and sufficient live feed density allowed a successful integration of B. calyciflorus into pikeperch larviculture. We hypothesize that feeding pikeperch larvae with a self-sustaining Brachionus-culture under pseudo-green water conditions with minor disruptions during larviculture will improve survival and growth. This system is a first step towards pikeperch larviculture inside recirculated aquaculture systems (RAS) under continuous feed supply with live feed within the same aquaculture unit.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
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  • 6
    Online Resource
    Online Resource
    MDPI AG ; 2017
    In:  Water Vol. 9, No. 7 ( 2017-07-11), p. 504-
    In: Water, MDPI AG, Vol. 9, No. 7 ( 2017-07-11), p. 504-
    Type of Medium: Online Resource
    ISSN: 2073-4441
    Language: English
    Publisher: MDPI AG
    Publication Date: 2017
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  • 7
    In: Fishes, MDPI AG, Vol. 4, No. 1 ( 2019-01-23), p. 3-
    Abstract: Ortho-phosphate inside recirculation aquaculture systems is limited as a consequence of precipitation and regular water exchange rates. To improve plant growth in coupled aquaponics, phosphate fertilizer addition to hydroponics can increase PO43−-P concentrations inside the process water. We investigated the effects of four PO43−-P concentrations ( 〈 10 (P0), 40, 80, 120 mg L−1) in rearing water on growth performance, feed efficiency, and welfare traits of juvenile African catfish (Clarias gariepinus Burchell, 1822). By trend, optimum specific growth rate of 2.66% d−1 and feed conversion ratio of 0.71 were observed at 40 and 80 mg L−1 PO43−-P. Higher PO43−-P significantly affected skin coloration, swimming activity and external injuries, with the palest and inactive fish combined with most external injuries in the P120 group. Mineral and protein contents in the fish remained unaffected, while fat content inside the fillets enriched with increasing PO43−-P. Inorganic P in blood plasma increased significantly, while phosphate concentrations inside the fillet remained unchanged. We suggest that PO43−-P concentrations of 40 to 80 mg L−1 do not reduce the performance of African catfish aquaculture, while increased values of 120 mg L−1 affect fish welfare. This allows limited addition of PO43−-P fertilizer in coupled aquaponics with African catfish to support plant growth.
    Type of Medium: Online Resource
    ISSN: 2410-3888
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
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  • 8
    In: Sustainability, MDPI AG, Vol. 14, No. 1 ( 2021-12-28), p. 305-
    Abstract: Mint (Mentha spicata) was cultivated in different hydroponic components: grow pipes, a raft and an ebb-and-flood gravel substrate system irrigated with aquaculture effluents from intensive African catfish (Clarias gariepinus) production under decoupled aquaponic conditions in northern Germany. The spearmint grew well and plant heights above ground were not significantly different between the gravel (57.7 ± 13.1 cm), raft (58.0 ± 17.7 cm) and grow pipe components (63.6 ± 9.9 cm). Root lengths and root fresh weights were two-fold and four-fold higher in raft (64.3 ± 20.5 cm; 42.8 ± 29.9 g) and grow pipes (59.4 ± 15.2 cm; 41.3 ± 25.7 g) compared with gravel substrate (29.7 ± 7.8 cm; 9.4 ± 9.4 g; raft = grow pipes 〉 gravel). Spearmint leaf number was significantly higher in the grow pipes (770.0 ± 224.4) than in the gravel substrate (499.8 ± 228.4) with intermediate values in the raft. Significantly highest mean fresh biomass was found in the raft (1275.6 ± 33.4 g), followed by grow pipes (1042.0 ± 35.8 g) and gravel substrate (686.3 ± 98.2 g; raft 〉 grow pipes 〉 gravel). M. spicata, under aquaponics, grows best in grow pipe and raft components. An increase in pipe diameter for the grow pipes and a reduction in the channel height for the raft components could optimize aquaponic culture conditions for both industrial production and the hobby sector in the future.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
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  • 9
    In: Agriculture, MDPI AG, Vol. 10, No. 10 ( 2020-10-11), p. 466-
    Abstract: Recycling of phosphorus (P) from feed input in aquaculture systems gains increasing importance, especially relating to sustainable agriculture and food production. In order to find possible areas of application of African catfish solid waste, the purpose of this study was to characterize the elemental and organic matter composition and P speciation in the aquaculture fish waste. Pyrolysis-field ionization mass spectrometry (Py-FIMS) was used to investigate the composition of organic matter and P K-edge X-ray absorption near edge structure (XANES) spectroscopy to describe the occurring P-containing compounds in African catfish solid waste from an intensive recirculation aquaculture system (RAS). The solid fish waste was mainly composed of sterols, free fatty acids and alkylaromatics, as it is common for digestive systems of animals. Ingredients such as the phytosterol beta-sitosterin confirm plant-based feed ingredients and some recalcitrance against digestion in the African catfish gut. The P in the solid fish waste was exclusively bound as calcium-phosphates. These calcium-phosphate minerals as major constituents of African catfish waste may have beneficial effects when applied to soils, suggesting the use of this waste as possible soil amendment in the future.
    Type of Medium: Online Resource
    ISSN: 2077-0472
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2651678-0
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  • 10
    In: AgriEngineering, MDPI AG, Vol. 4, No. 4 ( 2022-11-04), p. 1076-1094
    Abstract: In the present study, wheatgrass (Triticum aestivum L.) was chosen since fish effluents could be adequate to support its optimal growth. Wheatgrass was irrigated by water from African catfish (Clarias gariepinus) production under two stocking densities, namely extensive aquaculture unit (EAU) and intensive aquaculture unit (IAU), and tap water mixed with fertilizer (Control) in combination with three horticultural substrates: 100% coconut fibers (C), 70% coconut fibers + 30% perlite (CP), and 50% perlite + 50% vermiculite (PV) in an ebb-and-flow system. Different plant growth parameters, including nutrient contents, were evaluated and discussed. The results showed that regarding irrigation water, shoot dry mass was significantly higher in fish water groups. The root–shoot ratio was significantly higher in Control. The highest SPAD index was discovered in IAU. Regarding substrates, the root–shoot ratio was significantly low in C. Vitamin and mineral production reached mainly the highest concentrations in the combination of fish water with different substrates. In conclusion, irrigation water from IAU and coconut fibers were sufficient for optimal wheatgrass growth; meanwhile, IAU effluents showed a positive influence on vitamin production. Our study demonstrates the potential of aquaponics as a more sustainable way of producing superfoods.
    Type of Medium: Online Resource
    ISSN: 2624-7402
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
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