In:
Frontiers in Immunology, Frontiers Media SA, Vol. 11 ( 2021-1-25)
Abstract:
Spatial information of cells in their tissue microenvironment is necessary to understand the complexity of pathophysiological processes. Volumetric imaging of cleared organs provides this information; however, current protocols are often elaborate, expensive, and organ specific. We developed a simplified, cost-effective, non-hazardous approach for e fficient tissue clearing and m ulti- o rgan v olumetric i maging ( EMOVI ). EMOVI enabled multiplexed antibody-based immunolabeling, provided adequate tissue transparency, maintained cellular morphology and preserved fluorochromes. Exemplarily, EMOVI allowed the detection and quantification of scarce cell populations during pneumonitis. EMOVI also permitted histo-cytometric analysis of MHC-II expressing cells, revealing distinct populations surrounding or infiltrating glomeruli of nephritic kidneys. Using EMOVI, we found widefield microscopy with real-time computational clearing as a valuable option for rapid image acquisition and detection of rare cellular events in cleared organs. EMOVI has the potential to make tissue clearing and volumetric imaging of immune cells applicable for a broad audience by facilitating flexibility in organ, fluorochrome and microscopy usage.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2020.599495
DOI:
10.3389/fimmu.2020.599495.s001
DOI:
10.3389/fimmu.2020.599495.s002
DOI:
10.3389/fimmu.2020.599495.s003
DOI:
10.3389/fimmu.2020.599495.s004
DOI:
10.3389/fimmu.2020.599495.s005
DOI:
10.3389/fimmu.2020.599495.s006
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606827-8
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