Description: Salpa thompsoni is an important grazer in the Southern Ocean. It is found from the Subtropical Convergence southward to the coastal Antarctic Seas but being most abundant in the Antarctic Polar Frontal Zone. Low temperatures appear to negatively affect their development, limiting their ability to occur in the krill dominated high Antarctic ecosystems. Yet reports indicate that with ocean warming S. thompsoni have experienced a southward shift in their distribution. As they are efficient filter feeders, this shift can result in large-scale changes in the Southern Ocean ecosystem by increasing competitive or predatory interactions with Antarctic krill. To explore salp bloom dynamics in the Southern Ocean a size-structured S. thompsoni population model was developed with growth, consumption, reproduction and mortality rates dependent on temperature and chlorophyll a conditions. The largest uncertainties in S. thompsoni population ecology are individual and population growth rates, with a recent study identifying the possibility that the life cycle could be much shorter than previously considered. Here we run a suite of hypothesis scenarios under various environmental conditions to determine the most appropriate growth rate. Temperature and chlorophyll a were sufficient drivers to recreate seasonal and interannual dynamics of salp populations at two locations. The most suitable growth model suggests that mean S. thompsoni growth rates are likely to be ∼1mm body length d−1, 2-fold higher than previous calculations. S. thompsoni biomass was dependent on bud release time, with larger biomass years corresponding to bud release occurring during favorable environmental conditions; increasing the survival and growth of blastozooids and resulting in higher embryo release. This model confirms that it is necessary for growth and reproductive rates to be flexible in order for the salp population to adapt to varying environmental conditions and provides a framework that can examine how future salp populations might respond to climate change.

Description: The ongoing environmental changes in the Southern Ocean may cause a dramatic decrease in habitat quality. Due to its central position in the food web, Antarctic krill (Euphausia superba) is a key species of the marine Antarctic ecosystem. It is therefore crucial to understand how increasing water temperatures affect important krill life-cycle processes. Here, a long-term (August – March) laboratory acclimation experiment at different temperature scenarios (0.5 ◦C, 1.5 ◦C, 2.5 ◦C, 3.5 ◦C, 5 ◦C, 7 ◦C) was performed and the effects of elevated temperatures on whole animal parameters (O2 consumption, body length, length of the digestive gland) were analyzed. The response of krill oxygen consumption to different experimental temperatures differed between acute/short-term and long-term acclimation. After 8 months, krill oxygen consumption remained unchanged up to temperatures of 3.5 ◦C and was significantly higher at temperatures 〉 3.5 ◦C. Krill acclimated to temperatures ≥ 3.5 ◦C were significantly smaller at the end of the experiment. Limited food intake and/or conversion may have contributed to this effect, especially pronounced after the onset of the reproductive period. In addition, the seasonal growth pattern in males differed from that of females. Together, our findings indicate that warming Southern Ocean waters are likely to increase metabolic rate in krill, possibly altering the amount of energy available for other important life-cycle processes, a finding directly related to future population dynamics and fisheries management.

Description: Over the last decades, it has been reported that the habitat of the Southern Ocean (SO) key species Antarctic krill (Euphausia superba) has contracted to high latitudes, putatively due to reduced winter sea ice coverage, while salps as Salpa thompsoni have extended their dispersal to the former krill habitats. To date, the potential implications of this population shift on the biogeochemical cycling of the limiting micronutrient iron (Fe) and its bioavailability to SO phytoplankton has never been tested. Based on uptake of fecal pellet (FP)- released Fe by SO phytoplankton, this study highlights how efficiently krill and salps recycle Fe. To test this, we collected FPs of natural populations of salps and krill, added them to the same SO phytoplankton community, andmeasured the community’s Fe uptake rates. Our results reveal that both FP additions yielded similar dissolved iron concentrations in the seawater. Per FP carbon added to the seawater, 4.8 ± 1.5 times more Fe was taken up by the same phytoplankton community from salp FP than from krill FP, suggesting that salp FP increased the Fe bioavailability, possibly through the release of ligands. With respect to the ongoing shift from krill to salps, the potential for carbon fixation of the Fe-limited SO could be strengthened in the future, representing a negative feedback to climate change.

Description: The stomach content of 60 krill specimens from the Southern Ocean were analyzed for the presence of micro-plastic (MP), by testing different sample volumes, extraction approaches, and applying hyperspectral imaging Fourier-transform infrared spectroscopy (μFTIR). Strict quality control was applied on the generated results. A high load of residual materials in pooled samples hampered the analysis and avoided a reliable determination of putative MP particles. Individual krill stomachs displayed reliable results, however, only after re-treating the samples with hydrogen peroxide. Before this treatment, lipid rich residues of krill resulted in false assignments of polymer categories and hence, false high MP particle numbers. Finally, MP was identified in 4 stomachs out of 60, with only one MP particle per stomach. Our study highlights the importance of strict quality control to verify results before coming to a final decision on MP contamination in the environment to aid the establishment of suitable internationally standardized protocols for sampling and analysis of MP in organisms including their habitats in Southern Ocean and worldwide.

Description: Biological clocks are a ubiquitous ancient and adaptive mechanism enabling organisms to anticipate environmental cycles and to regulate behavioral and physiological processes accordingly [1]. Although terrestrial circadian clocks are well understood, knowledge of clocks in marine organisms is still very limited [2–5]. This is particularly true for abundant species displaying large-scale rhythms like diel vertical migration (DVM) that contribute significantly to shaping their respective ecosystems [6]. Here we describe exogenous cycles and endogenous rhythms associated with DVM of the ecologically important and highly abundant planktic copepod Calanus finmarchicus. In the laboratory, C. finmarchicus shows circadian rhythms of DVM, metabolism, and most core circadian clock genes (clock, period1, period2, timeless, cryptochrome2, and clockwork orange). Most of these genes also cycle in animals assessed in the wild, though expression is less rhythmic at depth (50–140 m) relative to shallow-caught animals (0–50 m). Further, peak expressions of clock genes generally occurred at either sunset or sunrise, coinciding with peak migration times. Including one of the first field investigations of clock genes in a marine species [5, 7], this study couples clock gene measurements with laboratory and field data on DVM. While the mechanistic connection remains elusive, our results imply a high degree of causality between clock gene expression and one of the planet’s largest daily migrations of biomass. We thus suggest that circadian clocks increase zooplankton fitness by optimizing the temporal trade-off between feeding and predator avoidance, especially when environmental drivers are weak or absent [8].