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  • Blackwell Publishing Ltd  (4)
Document type
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Three Acidophilic Volvocine Flagellates in Pure Culture (1964)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 11 (1964), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Three acidophilous volvocine flagellates from peat water in Czechoslovakia were obtained in pure culture by the washing technique. Two of these, Carteria acidicola and Chlamydomonas acidophila, grew so densely in low-pH Euglena gracilis media containing glutamic acid as to suggest they possess a vigorous heterotrophy. The other, Carteria turjosa, grew less well and required vitamin B12. In peat-water media the upper limit of growth was 5.5. The lowest pH tested, 2.5, permitted growth of Carteria acidicola and Chlamydomonas acidophila. Some peptone media permitted growth at pH 6.0. The morphology of these flagellates is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1964.tb01731.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Direct molecular biological analysis of ammonia oxidising bacteria populations in cultivated soil plots treated with swine manure (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 23 (1997), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The application of pig slurry, high in ammonia-nitrogen concentration, to agricultural land is a practice whose effect on soil microbial communities is poorly understood. The autotrophic ammonia-oxidising bacteria are an integral component of the nitrogen cycle in soil, and their activity will be affected by addition of nitrogenous fertilizer. Molecular biological techniques were applied to the direct detection and identification of ammonia-oxidiser populations in cultivated soil plots treated with different amounts of pig slurry. Members of the genus Nitrosospira were shown by 16S rDNA-directed PCR to be present in both unamended and amended soils, regardless of the quantity of pig slurry applied. In contrast, members of the genus Nitrosomonas were detected by the same approach only in those soil plots that had received high loadings of slurry. The fidelity of amplification products was always confirmed by oligonucleotide probing. In addition, we used high stringency PCR and confirmatory gene probing to detect the presence of the ammonia monooxygenase gene (amoA) of Nitrosomonas europaea directly in all amended soil samples, with hybridization signal intensities that increased with the amount of pig slurry applied to plots. Nitrosomonas europaea amoA DNA could not be detected in soil from the untreated plot. These data support the view that nitrosospiras are ubiquitous as important members of nitrifying populations in the environment. The direct detection of nitrosomonad DNA only in amended soils supports the hypothesis that these nitrosomonads become highly competitive under conditions analogous to laboratory enrichment cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1997.tb00390.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of soil improvement treatments on bacterial community structure and soil processes in an upland grassland soil (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 46 (2003), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Temporal temperature gradient electrophoresis (TTGE) analysis of 16S rRNA gene fragments amplified with primers selective for eubacteria and β-proteobacterial ammonia-oxidising bacteria (AOB) was used to analyse changes in bacterial and AOB community profiles of an upland pasture following soil improvement treatments (addition of sewage sludge and/or lime). Community structure was compared with changes in activity assessed by laboratory measurements of basal respiration and ammonia oxidation potentials, and with measurements of treatment- and time-related changes in soil characteristics. The predominant bacterial populations had a high degree of similarity under all treatment regimens, which was most pronounced early in the growing season. Most of the differences that occurred between soil samples with time could be accounted for by spatial and temporal variation; however, analysis of variance and cluster analysis of similarities between 16S rDNA TTGE profiles indicated that soil improvement treatments exerted some effect on community structure. Lime application had the greatest influence. The impact of soil improvement treatments on autotrophic ammonia oxidation was significant and sustained, especially in soils which had received sewage sludge and lime treatments in combination. However, despite obvious changes in soil characteristics, e.g. pH and soil nitrogen, increasing heterogeneity in the AOB community structure over time obscured the treatment effects observed at the beginning of the experiment. Nevertheless, time series analysis of AOB TTGE profiles indicated that the AOB community in improved soils was more dynamic than in control soils where populations were found to be relatively stable. These observations suggest that the AOB populations exhibited a degree of functional redundancy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0168-6496(03)00160-0
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Survival of a Shiga toxin-encoding bacteriophage in a compost model (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 245 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bacteriophages that carry the Shiga toxin gene (stx) represent an additional hazard in cattle manure-based fertilizers in that their survival could lead to toxigenic conversion of Escherichia coli and other bacteria post-composting. A Stx-phage in which the Shiga toxin (stx2) gene was inactivated by insertion of a chloramphenicol resistance gene was used in combination with a rifampicin-resistant E. coli host where RecA is constitutively activated so that all infectious phage particles could be enumerated by plaque assay. PCR-based confirmation methods and the additional application of a host enrichment protocol ensured that very low numbers of surviving bacteriophage could be detected and unequivocally identified. Stx-bacteriophage numbers declined rapidly over the first 48 h and none could be detected after 3 days. The host enrichment method was applied after 6 days and no bacteriophages were recovered. While addition of fresh E. coli cells at intervals after the compost temperature had reduced below 40 °C demonstrated that E. coli growth could be supported in the compost, Stx-phages or their lysogens were never detected. Here, we demonstrate that composting animal manure for 40 days during which a temperature of 〉60 °C is maintained for at least 5 days is effective at removing both E. coli and a model infectious Stx-encoding bacteriophage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.03.031
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    Paper (German National Licenses)
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