In:
American Journal of Physiology-Endocrinology and Metabolism, American Physiological Society, Vol. 280, No. 1 ( 2001-01-01), p. E187-E192
Abstract:
The purpose of the present study was to evaluate a novel approach for determining skeletal muscle-specific glucose flux using radioactive stereoisomers and the microdialysis technique. Microdialysis probes were inserted into the vastus lateralis muscle of human subjects and perfused (4 μl/min) with a Ringer solution containing small amounts of radioactived- and l-glucose as the internal reference markers for determining probe recovery as well as varying concentrations of insulin (0–10 μM). The rationale behind this approach was that both stereoisomers would be equally affected by the factors that determine probe recovery, with the exception ofl-glucose, which is nonmetabolizable and would not be influenced by tissue uptake. Therefore, any differences in the probe recovery ratios between the d- andl-stereoisomers represent changes in skeletal muscle glucose uptake directly at the tissue level. There were no differences in probe recovery between the d- (42.3 ± 3.5%) andl- (41.2 ± 3.5) stereoisomers during the control period (no insulin), which resulted in a D/L ratio of 1.04 ± 0.03. However, during insulin perfusion (1 μM), The D/L ratio increased to 1.62 ± 0.08 and 1.58 ± 0.07 ( P 〈 0.05) during the two collection (0–15 and 15–30 min) periods, respectively. This was accomplished solely by an increase ( P 〈 0.05) in d-glucose probe recovery, asl-glucose probe recovery remained unchanged. In a second set of experiments, the perfusion of 10 μM insulin did not increase the D/L ratio (1.40 ± 0.11) above that observed during 1.0 μM (1.41 ± 0.07) insulin perfusion. These data suggest that this method is sufficiently sensitive to detect differences in insulin-stimulated glucose uptake; thus the use of radioactive stereoisomers in conjunction with the microdialysis technique provides a novel and useful technique for determining tissue-specific glucose flux and insulin sensitivity.
Type of Medium:
Online Resource
ISSN:
0193-1849
,
1522-1555
DOI:
10.1152/ajpendo.2001.280.1.E187
Language:
English
Publisher:
American Physiological Society
Publication Date:
2001
detail.hit.zdb_id:
1477331-4
SSG:
12
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