In:
Clinical Cancer Research, American Association for Cancer Research (AACR), Vol. 16, No. 14_Supplement ( 2010-07-15), p. B25-B25
Abstract:
Two consecutive experiments were conducted for understanding immunity, stemness, and tumorigenesis of the embryonic stem cells (ESCs) in their allograft. In the first series of experiment, we evaluated whether co-injection of the stem cells with somatic cells could evade the immune response. Donor B6CBAF1 mouse ESCs were subcutaneously co-injected either alone or with ovarian stromal cells, fetal fibroblasts, or adult fibroblasts isolated from the recipient B6D2F1 mouse strain. ESCs by themselves induced teratomas (14%). However, when somatic cells were injected together, ESC-mediated teratoma formation significantly increased (33-75%). Especially, the highest rate of the teratoma formation was observed in ESCs and fetal fibroblasts co-injected recipient B6D2F1 mice (75%). Next, we derived ESC-like, cancer stem cells from the teratomas with enzymatic digestion and their differentiation potential was subsequently evaluated. Except for one cell line, other all cell lines showed a similar morphology to that of original ESCs and retained alkaline phosphatase activity, ESC-specific gene expression (Oct-4, Nanog, Cripto, and Rex-1), and Oct-4 protein expression regardless of their increased abnormality in chromosome number. Relative quantification of lineage-specific gene (Nestin, SMA, Desmin, and Krt8) expression of teratoma-derived cell lines showed the significant changes between the ESCs and the teratoma-derived, ESC-like cells derived from the cell co-injection. Variations in the type of co-injected cells led to lineage-specific changes, changes in gene expression in isolated colony-forming cells, and alterations in stem-cell-like cells derived from allografted tissues. In conclusion, our results suggest that cell-to-cell interaction regulates cellular immunity for implanted cells, which further affects tumorigenesis and stemness [Supported by a grant (SC-5160) from Stem Cell Research Center of the 21st Century Frontier Research Program awarded by Jeong-Mook Lim] . Jong-Heum Park and Seo-Jin Oh contributed equally to this work. Citation Information: Clin Cancer Res 2010;16(14 Suppl):B25.
Type of Medium:
Online Resource
ISSN:
1078-0432
,
1557-3265
DOI:
10.1158/1078-0432.TCMUSA10-B25
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2010
detail.hit.zdb_id:
1225457-5
detail.hit.zdb_id:
2036787-9
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