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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 19 (1972), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— Multiple forms of calf brain N-acetyl-β-hexosaminidases (β-2-acetamido-2- deoxy-d-glucoside acetamidodeoxyglucohydrolase EC 3.2.1.30) were separated on starch gel electrophoresis at pH 5.8. The organ specific electrophoretic patterns did not depend on the cell fraction studied. Much of the activity is only separated with difficulty from particulate matter. Two major and one minor component were separated on DEAE-cellulose chromatography at pH 5.8. Each component had both N-acetyl-β-galactosaminidase and N-acetyl-β-glucosaminidase activity. The ratio of these two activities was unaffected by the presence of N-ethylmaleimide or dithiothreitol. The forms were also examined by isoelectric focusing when at least four components were recognized: isoelectric at 4.9, 6.0, 6.3 and 6.8. Interconversion of the 4.9 form to that isoelectric at pH 6.0 was noted during vacuum dialysis. Samples from normal human brain and from cases of Tay-Sachs disease were also examined and the results compared.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 5 (1972), S. 248-249 
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: Preparation method, lattice parameters and powder data are given. Space group Ama2 was confirmed and and x and y atomic parameters were determined from Weissenberg photographs.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 30 (1974), S. 1470-1473 
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 246 (1973), S. 155-156 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Structures variously called Querbalken, crossbands, and annuli have been reported in Caulobacter stalks since they were first observed in the electron microscope8-6. This study was undertaken to determine how the formation of these structures is related to the life cycle of the bacterium. ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 96 (1974), S. 1-16 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Prosthecae were sheared from cells ofAsticcacaulis biprosthecum with a Sorvall omnimixer and purified by centrifugation. Removal of prosthecae resulted in no loss in viability. Purified prosthecae contain protein, carbohydrates and lipids. Certain enzymes that are present in the main body of the cell are also present in prosthecae: malic dehydrogenase (E.C. 1.1.1.37), alkaline phosphatase (E. C. 3.1.3.1), succinic dehydrogenase (E.C. 1.3.99.1), cytochromec reductase (E.C. 1.6.2.a), and cytochromes. Other enzymes present in whole cells are not in prosthecae: isocitric dehydrogenase (E.C. 1.1.1.41), NADH oxidase (E.C. 1.6.99.3), glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49), and lactic dehydrogenase (E.C. 1.1.2.3). Swarmer cells (non-prosthecate) were purified by allowing prosthecate cells to attach to cheesecloth in the growth medium. Envelopes prepared from prosthecae were compared with envelope from swarmer cells. The two envelope fractions differed in the disc-gel electrophoretic patterns of proteins solubilized from them.
    Type of Medium: Electronic Resource
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