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  • 1
    Electronic Resource
    Electronic Resource
    Spectroscopic properties, electronic levels, and crystal field parameters of pentakis(nitrato)europate(III) ions (1983)
    s.l. : American Chemical Society
    Inorganic chemistry 22 (1983), S. 3763-3768 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00167a018
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  • 2
    Electronic Resource
    Electronic Resource
    Induction of Human T Colony Formation by Phorbol Myristate Acetate (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 17 (1983), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Phorbol myristate acetate (PMA) is a potent inducer of T colony formation by peripheral blood lymphocytes. A mean cloning efficiency of 0.3% (0.05-0.5%) is obtained with PMA concentrations of 100-1000 ng/ml. PMA-induced T colony formation does not require the presence of monocytes and therefore differs from other mitogens in this respect. Purified T-colony-promoting activity (TCPA) (devoid of phytohaemagglutinin (PHA)) increases PMA-induced T colony numbers and induces T colony formation at low PMA doses (0.01 to 1 ng), concentrations at which no T colonies are detected in the absence of added TCPA. PMA-induced colonies are mainly composed of cells bearing Fc receptors for IgM (54%), which is not the case for colonies obtained with PHA (11 %). PMA-induced colony cells do not bind OKT3 and OKT4 monoclonal antibodies, whereas 23% are able to bind OK-T8 antibody. These results demonstrate that PMA is a potent inducer of T colony formation and may therefore serve as a useful tool for the study of T-cell differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1983.tb00797.x
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  • 3
    Electronic Resource
    Electronic Resource
    Ascorbic Acid and Vitamin A Activity in Selected Vegetables from Different Geographical Areas of the United States (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Reduced ascorbic acid (RAA) and Vitamin A (carotenoid) contents of six vegetables obtained from six cities in the United States at two seasons of the year were determined. Mean RAA content (mg/100g) of cabbage was 45.2, carrots 7.8, celery 6.0, corn 6.5, onions 8.4, and tomatoes 15.3. Vitamin C in cooked cabbage was 22.1, corn 6.2, and onions 5.7 mg/100g. Mean vitamin A activity (I.U.) of carrots was 15,228, cabbage 114, celery 133, corn 219, and tomatoes 750. In cooked cabbage and corn, vitamin A content was 89 and 217 I.U., respectively. The RAA and vitamin A content of vegetables from the six geographical areas varied significantly. Vitamin concentrations were generally lower than tabulated values. Retention of RAA in cooked cabbage was 52%; in onions, 58%; and in corn, 98%. Vitamin A retention in cooked cabbage was 82%, and in cooked corn was 98%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1982.tb12750.x
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  • 4
    Electronic Resource
    Electronic Resource
    Comparison Between Sensory Quality of Freshly Prepared Spaghetti with Meat Sauce Before and After Hot Hording on a Cafeteria Counter (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 49 (1984), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sensory quality of freshly prepared spaghetti with meat sauce entree was compared to that held hot on a cafeteria counter. A study of the time and temperature relationship indicated a steady decline in temperatures during the first 30 min of steam-table holding, although within safe limits. An experienced taste panel consisting of 8–10 members rated the samples for various sensory attributes. The freshly cooked product was rated significantly higher compared to the hot-held product for moistness, appearance of spaghetti, intensity of spice flavor in the sauce, spaghetti texture, meat texture, and general acceptability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1984.tb12824.x
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  • 5
    Electronic Resource
    Electronic Resource
    Auto-Delayed-Type Hypersensitivity Induced in Immunodeficient Mice with Modified Self-Antigens (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 20 (1984), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Suppressor cells obtained from spleens of normal A mice, or factor extracted from these suppressor cells, abolished the syngeneic delayed-type hypersensitivity (syn-DTH) response of X-irradiated A mice injected with trinitrophenylated spleen cells und challenged with syngeneic lymphoblasts. Some of the physical, chemical and biological properties of the suppressive factor (SF) were characterized. The SF was relatively temperature-stable and its activity was destroyed by pronase (but not with RNase or DNase). The activity of the SF was absorbed on concanavalin A and anti-I-Jk Scpharose columns, suggesting that the factor is a glycoprotein-bearing I-Jk product. The approximate molecular weight of the factor is 50,000–60,000. The SF was absorbed on plastic adherent cells (but not on non-adherent cells). Adherent cells that absorbed the SF abrogated the ability of primed T cells to transfer the syn-DTH to naive X-irradiated recipients. In contrast, SF that was presented directly to the primed T cells failed to abolish their ability to transfer DTH. These findings suggest that the adherent cells serve as mediators, transferring the SF from factor-producing cells (Lyt-1+2+3+, I-Jk+ T cells) to target cells (Lyt-1+ primed T cells).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1984.tb01019.x
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  • 6
    Electronic Resource
    Electronic Resource
    Partial Replacement of Wheat Flour with Yellow Field Pea Flour in White Pan Bread (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Yellow field pea flour replaced 2.5, 5 and 10% of wheat flour in white pan bread prepared by a straight dough, rapid mix home formulation. Incorporation of pea flour did not affect proofing times. No significant differences were found between Gardner L and “a” values for wheat-pea flour blends, but “b” values increased with increasing levels of pea flour; crumb color of breads did not differ significantly. Volumes of the breads were not significantly affected by pea flour addition. Pea flour breads were judged between fair and good for all characteristics by an experienced taste panel; the 10% pea flour breads were scored significantly lower for texture, color and flavor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1982.tb11092.x
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  • 7
    Electronic Resource
    Electronic Resource
    Folacin and Ascorbic Acid Retention in Fresh Raw, Microwave, and Conventionally Cooked Spinach (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fresh spinach was assayed for total folacin and reduced ascorbic acid content, before and after cooking by a microwave and conventional method. Mean ascorbic acid content of raw, microwave cooked and conventionally cooked spinach was 26.5 mg/lOOg, 13.5 mg/l00g, and 17.0 mg/lOOg, respectively. Ascorbic acid content was decreased significantly by both cooking methods; retention was 47% in microwave and 51% in conventionally cooked spinach. Total folacin content of raw, microwave, and conventionally cooked spinach was 161 mcg/lOOg, 183 mcg/lOOg, and 157 mcg/lOOg, respectively. Retention of folate was 77% in conventionally cooked and 101% in microwave cooked spinach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1981.tb04931.x
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  • 8
    Electronic Resource
    Electronic Resource
    Immunogenicity of subcellular fractions and molecular species of MuLV-induced tumors (1982)
    Springer
    Cancer immunology immunotherapy 14 (1982), S. 16-26 
    Details
    ISSN: 1432-0851
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary YBA, a Moloney virus-induced leukemia in CBA mice, and a relatively weak immunogenic tumor, was screened for the presence of immunogenic antigens. The tumor was subjected to homogenization and subcellular fractionation on sucrose gradients; the immunogenic subcellular fractions underwent further separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The immunogenicity of the subcellular fractions and the SDS-PAGE-isolated molecular species were tested by (their) subcutaneous injection into syngeneic mice and examination of their splenocytes examined against tumor cell and normal cell targets by the chromium release cell-mediated lympholysis assay. Tumor cell homogenates were also separated by SDS-PAGE and tested for immunogenicity without prior fractionation. Splenocytes from mice that had received injections of certain SDS-PAGE-isolated epitopes derived from YBA tumor homogenate or its light and heavy subcellular fractions generated effective cytotoxic responses against YBA target cells after 6 days in vitro cultivation. In contrast, intact YBA tumor cells or non-separated tumor homogenates failed to induce an efficient cytotoxic response. The effector cells induced with the immunogenic SDS-PAGE-isolated epitopes of YBA tumor were specific, since they cytolysed the homologous target cells more efficiently than unrelated target cells or syngeneic normal cells. The activity of these effector cells was affected by varying the effector : target ratio. Augmentation of the cytotoxic responses was obtained when the splenocytes of mice immunized with SDS-PAGE-isolated epitopes of YBA tumor were restimulated in vitro, with the homologous neoplastic cells. Immunogenic SDS-PAGE epitopes were isolated from YAC tumor also (YAC is a Moloney-induced tumor of A mice). The effector cells induced with these separated epitopes were characterized as thymus-derived cells and not as natural killer cells. The results suggest that (1) the molecular repertoire of YBA and YBA tumors contain immunogens that can induce a specific antitumor cell-mediated response; (2) the isolated molecular species injected are more efficient immunogens than the entire, unseparated homogenate sample or a dose of 108 intact inactivated tumor cells; and (3) the gel matrix may be responsible for the enhanced cell-mediated response induced against the weakly immunogenic tumor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199427
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  • 9
    Electronic Resource
    Electronic Resource
    Amino acid, ammonia and neurotransmitter concentrations in hepatic encephalopathy: Serial analysis in plasma and cerebrospinal fluid during treatment with an adapted amino acid solution (1984)
    Springer
    Journal of molecular medicine 62 (1984), S. 867-875 
    Details
    ISSN: 1432-1440
    Keywords: Hepatic encephalopathy ; Amino acids ; Neurotransmitters in cerebrospinal fluid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We evaluated changes of advanced liver disease and hepatic encephalopathy on the concentrations of amino acids (AA) and ammonia in plasma and cerebrospinal fluid (CSF) and of the neurotransmitters norepinephrine, dopamine and 5-hydroxytryptamine (5-HT) as well as the 5-hydroxyindole acetic acid (5-HIAA) in CSF before and at the end of a 3-day period of treatment with infusions enriched with branched chain amino acids (BCAA). The subjects studied were 13 patients with alcoholic cirrhosis and hepatic encephalopathy stages 1–3 (n=8) and stage 4 (n=5). The patients in coma stages 1–3 recovered during the treatment (survivors), those in coma stage 4 died before the study period was finished (non-survivors). The data emerging from this study show: 1. Alterations of AA concentrations are much more pronounced in the CSF than in the plasma. In the case of tryptophan the alterations in plasma and CSF were inverse. 2. Before the treatment the CSF-plasma ratios of the concentrations of BCAA and aromatic amino acids (AAA) are increased reflecting an activated transport of both the BCAA and AAA through the blood-brain barrier. 3. High dose BCAA nearly normalized CSF concentrations and CSF-plasma ratios of AAA assuming that the treatment brought about an effective competition of cerebral uptake between BCAA and AAA. 4. The CSF concentrations of ammonia and glutamine decreased significantly during treatment while the plasma concentrations changed only moderately. 5. As to the neurotransmitters, only the concentrations of 5-HT and its metabolite 5-HIAA correlated with the clinical picture and with the concentration of their precursor AA. 6. The normalization or improvement of the CSF concentrations of the AAA, ammonia and glutamine as well as the neurotransmitter 5-HT were accompanied by an improvement of hepatic encephalopathy stages 1–3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01712006
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  • 10
    Electronic Resource
    Electronic Resource
    Transformations of inorganic nitrogen by Azospirillum spp. (1981)
    Springer
    Archives of microbiology 130 (1981), S. 96-100 
    Details
    ISSN: 1432-072X
    Keywords: Nitrogen fixation ; Nitrate respiration ; Denitrification ; Assimilatory nitrate reduction ; Dissimilatory nitrate reduction ; Acetylene reduction ; Azospirillum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Azospirillum spp. participate in all steps of the nitrogen cycle except nitrification. They can fix molecular nitrogen and perform assimilatory nitrate reduction and nitrate respiration. Culture conditions have been defined under which nitrate is used both as terminal respiratory electron acceptor and as nitrogen source for growth. Nitrate and, possibly to a very limited extent, nitrite, but not sulfate, iron or fumarate support anaerobic respiration. Under anaerobic conditions, nitrate can also supply energy for nitrogen fixation but without supporting growth. Nitrate-dependent nitrogenase activity lasts only for 3–4 h until the enzymes of assimilatory nitrate reduction are synthesized. Nitrite accumulates during this period and inhibits nitrogenase activity at concentrations of about 1 mM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411058
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