In:
FEBS Letters, Wiley, Vol. 172, No. 2 ( 1984-07-09), p. 326-330
Abstract:
Cells of Dictyostelium discoideum were incubated during the preaggregation phase with [ 3 H] palmitic acid, and association of the fatty acid label with proteins was investigated by SDS‐polyacrylamide gel electrophoresis and fluorography. Three proteins with apparent molecular masses of 44, 80 and 130kDa were preferentially labeled. The 80‐kDa protein proved to be identical with an integral membrane glycoprotein called contact site A. The protein retained the 3 H label after chloroform‐methanol extraction and boiling in SDS‐containing buffer, but not after mild hydroxylamine treatment, indicating an ester bond. After treatment of the protein with KOH the 3 H label was recovered in free palmitic acid, as demonstrated by gas‐liquid chromatography of the methyl ester.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(84)81150-3
Language:
English
Publisher:
Wiley
Publication Date:
1984
detail.hit.zdb_id:
1460391-3
SSG:
12
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