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  • OceanRep  (9)
  • 1980-1984  (9)
  • 1
    Publication Date: 2016-01-22
    Description: Seven strains of five species of the genus Ectothiorhodospira were characterized by oligonucleotide cataloguing of their 16S rRNA in order to determine the phylogenetic relationship to one another and to other phototrophic purple bacteria. All representatives of Ectothiorhodospira are members of that line of descent defined by phototrophic purple sulfur bacteria and relatives, showing a moderate relationship to those phototrophic organisms forming globules of elemental sulfur inside the cell (Chromatium and relatives). The 5 Ectothiorhodospira species fall into two subgroups. E.halophila, E. halochloris and E. abdelmalekii form one, E. mobilis, E. shaposhnikovii and the unnamed strain BN 9906 form the second subgroup. Within the two subgroups the strains are closely related, while the degree of relatedness found between members of the two subgroups is more distant.
    Type: Article , PeerReviewed
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  • 2
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    American Society for Microbiology
    In:  Journal of Bacteriology, 160 (1). pp. 278-479.
    Publication Date: 2016-01-22
    Description: A number of moderately halophilic bacteria of diverse taxonomic groups have been studied to determine the intracellular concentrations of organic compounds at various salt concentrations. Betaine was accumulated in all of these organisms in proportion to the salinity of the medium, suggesting that this compound plays a major role in osmoregulation.
    Type: Article , PeerReviewed
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  • 3
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    Springer
    In:  Archives of Microbiology, 130 (3). pp. 234-237.
    Publication Date: 2016-01-21
    Description: Rhodopseudomonas globiformis is able to grow on sulfate as sole source of sulfur, but only at concentrations below 1 mM. Good growth was observed with thiosulfate, cysteine or methionine as sulfur sources. Tetrathionate supported slow growth. Sulfide and sulfite were growth inhibitory. Growth inhibition by higher sulfate concentrations was overcome by the addition of O-acetylserine, which is known as derepressor of sulfate-assimilating enzymes, and by reduced glutathione. All enzymes of the sulfate assimilation pathway. ATP-sulfurylase, adenylylphosphate-sulfotransferase, thiosulfonate reductase and O-acetylserine sulfhydrylase are present in R. globiformis. Sulfate was taken up by the cells and the sulfur incorporated into the amino acids cysteine, methionine and homocysteine. It is concluded, that the failure of R. globiformis to grow on higher concentrations of sulfate is caused by disregulation of the sulfate assimilation pathway. Some preliminary evidence for this view is given in comparing the activities of some of the involved enzymes after growth on different sulfur sources and by examining the effect of O-acetylserine on these activities.
    Type: Article , PeerReviewed
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  • 4
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    Urban & Fischer
    In:  Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ökologische Mikrobiologie, 1 (1). pp. 61-69.
    Publication Date: 2015-03-24
    Description: Enrichment cultures inoculated with fragments of the sponge Ircinia spec, under autotrophic culture conditions and with thiosulfate as sole electron donor yielded the predominant development of a small cell Chromatium strain, which is described herein as the new species Chromatium purpuratum. Autotrophically grown cells are 1.2–1.7 μm wide and 3–4 μm long. The cells are motile by means of one single polar flagellum. Intracytoplasmic membranes are present as vesicles as in the other Chromatium species. Multiplication occurs by binary fission. The photopigments are bacteriochlorophyll a and carotenoids of the okenone series. The G + C content of the type strain BN 5500 is 68.9 mole%. Chromatium purpuratum grows well photoautotrophically with sulfide or thiosulfate as electron donor. Photoheterotrophic growth is possible with various fatty acids serving as electron donor and carbon source. The new species is a marine isolate with an optimal salinity of 5% NaCl. It is compared with other known species of the genus Chromatium
    Type: Article , PeerReviewed
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  • 5
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    Urban & Fischer
    In:  Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ökologische Mikrobiologie, 2 (3). pp. 228-234.
    Publication Date: 2015-03-24
    Description: A new species of the genus Ectothiorhodospira is described isolated from alkaline soda lakes of the Wadi Natrun in Egypt. In most of its properties it resembles the recently described Ectothiorhodospira halochloris, which had been isolated from similar lakes of the same area. The new species, Ectothiorhodospira abdelmalekii, has spiral-shaped cells with 0.9-1.2 μm diameter, it is polarly flagellated with sheated flagella, has lamellar photosynthetic membrane stacks, possesses bacteriochlorophyll b as the main photosynthetic pigment, and has a DNA base composition of 63.3–63.8 mol% G + C. Photolithotrophic growth with sulfide or elemental sulfur as electron donors is the predominant mode of life. During sulfide oxidation to sulfate, elemental sulfur is accumulated outside the cells. Acetate, pyruvate, fumarate and succinate are photoassimilated. The properties of E. halochloris and E. abdelmalekii are compared.
    Type: Article , PeerReviewed
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  • 6
    Publication Date: 2020-07-24
    Description: The amino acid composition of total proteins of eight Ectothiorhodospira strains with different salt optima and of separated membranes of selected strains have been determined. Amino acid compositions were compared with those reported for nonhalophilic phototrophic and heterotrophic bacteria and Halobacterium halobium. The membrane fractions from Ectothiorhodospira strains requiring high salt for maximum growth contained more polar and less nonpolar amino acids than strains with low salt requirements or nonhalophilic bacteria. The content of intermediate amino acids increased with the increasing halophilic properties of the Ectothiorhodospira strains. Proteins which function in high-salt environments may therefore require such compositions to maintain their structures in highly ionic solutions.
    Type: Article , PeerReviewed
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  • 7
    Publication Date: 2016-01-22
    Description: A new purple nonsulfur bacterium was isolated from enrichment cultures of a sulfide-containing marine lagoon. The bacterium is similar to Rhodopseudomonas capsulata and is described as a new species of the genus Rhodopseudomonas: Rhodopseudomonas adriatica. Cells are non-motile, 0.5–0.8 μm by 1.3–1.8 μm, and multiply by binary fission. Intracytoplasmic membranes are of the vesicular type. The photosynthetic pigments are bacteriochlorophyll a and carotenoids of the spheroidene group. Growth is possible anaerobically in the light and at low pO2 in the dark. Biotin and thiamine are required as growth factors. A wide variety of organic compounds, as well as sulfide and thiosulfate, are used as photosynthetic electron donors. Sulfide is oxidized to elemental sulfur, which is subsequently converted to sulfate, whereas thiosulfate oxidation occurs without measurable intermediate. Rhodopseudomonas adriatica is unable to assimilate sulfate, growth is only possible in the presence of a reduced sulfur compound.
    Type: Article , PeerReviewed
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  • 8
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    Springer
    In:  Archives of Microbiology, 132 (2). pp. 197-203.
    Publication Date: 2016-01-21
    Description: The ability to use adenosine 5′-phosphosulfate (APS) or 3′-phosphoadenosine 5′-phosphosulfate (PAPS) as the substrate for the initial reductive step in sulfate assimilation has been tested in most of the known Rhodospirillaceae species and in some chemotrophic bacteria. Improved and optimized methods for the synthesis and purification of the sulfonucleotides APS and PAPS are described. The production of acid volatile radioactivity from 35S-APS and 35S-PAPS was measured under various conditions in the presence and absence of non-labeled sulfate. Specific differences in the ability to reduce APS or PAPS were observed among the Rhodospirillaceae species and also the chemotrophic bacteria. APS was found to be the substrate of the thiolsulfotransferase in Rps. acidophila, Rps. globiformis, Rm. vannielii, Rc. purpureus, R. tenue, Rps. gelatinosa, in Alcaligenes eutrophus and Pseudomonas aeruginosa. PAPS was the substrate in Rps. capsulata, Rps. sphaeroides, Rps. sulfidophila, Rps. palustris, Rps. viridis, R. rubrum, R. fulvum, in Paracoccus denitrificans and in several Enterobacteriaceae. The presence of different enzymatic systems for sulfate reduction in the Rhodospirillaceae family is compared with their taxonomical grouping and their possible phylogenetic relatedness.
    Type: Article , PeerReviewed
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  • 9
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    Springer
    In:  Archives of Microbiology, 136 (2). pp. 96-101.
    Publication Date: 2016-01-21
    Description: The mechanism of sulfate assimilation was investigated in Rhodopseudomonas sulfidophila, a bacterium able to grow either photoautotrophically, with sulfide as electron donor, or photoheterotrophically with sulfate as sole sulfur source. ATP sulfurylase, adenosine-5′-phosphosulfate kinase, 3′-phosphoadenosine-5′-phosphosulfate sulfotransferase, thiosulfonate reductase and cysteine synthase were present. Reduced sulfur compounds, especially sulfide and sulfite repressed all steps of sulfate activation and reduction including sulfate uptake. Adenosine-5′-phosphosulfate kinase activity in contrast to the other activities was high in the presence of cysteine or reduced glutathione in the growth medium. Sulfur was incorporated into the cellular sulfolipid from sulfate and also from reduced sulfur compounds like cysteine and thiosulfate. The activity of 3′-phosphoadenosine-5′-phosphosulfate sulfotransferase was rapidly lost during gel filtration or dialysis. From comparison with other sulfotransferases and from the specific cofactor requirement for the enzyme of R. sulfidophila it is concluded that two different low molecular weight cofactors are required in this system. A reaction sequence is proposed involving thioredoxin as the reductant of another dialysable low molecular weight cofactor, that binds to the protein.
    Type: Article , PeerReviewed
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