In:
Cytometry, Wiley, Vol. 10, No. 3 ( 1989-05), p. 263-272
Abstract:
The authors have developed a new methodology for characterizing in situ the cell cycle of human mammary epithelial cell lines. Using a SAMBA 200 cell image processor (scanning cytometry), 15 densitometric and textural parameters were computed on each Feulgen‐stained nucleus. Parameters computed from the grey level cooccurrence and run‐length section matrices allowed assessment of the chromatin pattern. Multiparametric analysis of data defined: (1) the relative position of each cell; (2) the relative positions of groups of cells, each group corresponding to a definite phase of the cell cycle; and (3) the function of these parameters best separating these phases. Files then were constructed for each phase: G 0 /G 1 , S, G 2 / and M. Using these three files as a reference to classify cells, it was possible to ascertain the phase of the cell cycle for each cell of a population. The MDA AG human cell line synchronized by mitotic selection was used as a model to develop this method. The criteria used to assign cells to G 0 /G 1 , S, or G 2 was DNA content. Classification in M phase was achieved by visual identification of mitotic cells. This method was checked on unsynchronized MDA AG and then applied to other human cell lines (MDA MB231, MCF‐7, T47D C111). Comparison of results obtained by scanning cytometry and flow cytometry showed the proportion of cells assigned to G 0 /G 1 , S, and G 2 /M by the two methods to be similar. This new method removes some of the limitations of flow cytometry by (1) allowing visual verification of each cell analyzed; (2) lowering the number of cells required for study; (3) discriminating between G 2 and M; and (4) preserving cell topography.
Type of Medium:
Online Resource
ISSN:
0196-4763
,
1097-0320
DOI:
10.1002/cyto.990100305
Language:
English
Publisher:
Wiley
Publication Date:
1989
detail.hit.zdb_id:
2180639-1
detail.hit.zdb_id:
1474272-X
detail.hit.zdb_id:
2180651-2
SSG:
12
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