In:
Kidney and Blood Pressure Research, S. Karger AG, Vol. 42, No. 6 ( 2017), p. 1078-1089
Abstract:
〈 b 〉 〈 i 〉 Background/Aims: 〈 /i 〉 〈 /b 〉 To date, there is no imaging technique to assess tubular function 〈 i 〉 in vivo 〈 /i 〉 . Blood oxygen level-dependent magnetic resonance imaging (BOLD MRI) measures tissue oxygenation based on the transverse relaxation rate (R2*). The present study investigates whether BOLD MRI can assess tubular function using a tubule-specific pharmacological maneuver. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Cross sectional study with 28 participants including 9 subjects with ATN-induced acute kidney injury (AKI), 9 healthy controls, and 10 subjects with nephron sparing tumor resection (NSS) with clamping of the renal artery serving as a model of ischemia/reperfusion (I/R)-induced subclinical ATN (median clamping time 15 min, no significant decrease of eGFR, p=0.14). BOLD MRI was performed before and 5, 7, and 10 min after intravenous administration of 40 mg furosemide. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Urinary neutrophil gelatinase-associated lipocalin was significantly higher in ATN-induced AKI and NSS subjects than in healthy controls (p=0.03 and p=0.01, respectively). Before administration of furosemide, absolute medullary R2*, cortical R2*, and medullary/cortical R2* ratio did not significantly differ between ATN-induced AKI vs. healthy controls and between NSS-I/R vs. contralateral healthy kidneys (p & #x3e;0.05 each). Furosemide led to a significant decrease in the medullary and cortical R2* of healthy subjects and NSS contralateral kidneys (p & #x3c;0.05 each), whereas there was no significant change of R2* in ATN-induced AKI and the NSS-I/R kidneys (p & #x3e;0.05 each). 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 BOLD-MRI is able to detect even mild tubular injury but necessitates a tubule-specific pharmacological maneuver, e.g. blocking the Na 〈 sup 〉 + 〈 /sup 〉 -K 〈 sup 〉 + 〈 /sup 〉 -2Cl 〈 sup 〉 - 〈 /sup 〉 transporter by furosemide.
Type of Medium:
Online Resource
ISSN:
1420-4096
,
1423-0143
Language:
English
Publisher:
S. Karger AG
Publication Date:
2017
detail.hit.zdb_id:
1482922-8
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