In:
Frontiers in Cell and Developmental Biology, Frontiers Media SA, Vol. 9 ( 2021-4-21)
Abstract:
NSUN5 , encoding a cytosine-5 RNA methyltransferase and located in the 7q11.23 locus, is a candidate gene for tetralogy of Fallot (TOF). Deletion of the 7q11.23 locus in humans is linked to cardiac outflow tract (OFT) disorders including TOF. We identified four potential pathogenic mutations in the coding region of NSUN5 and which were enriched in TOF patients by an association study of 132 TOF patients and 2,000 in-house controls ( P = 1.44 × 10 –5 ). We then generated a Nsun5 null ( Nsun5 –/– ) mouse model to validate the human findings by defining the functions of Nsun5 in OFT morphogenesis. The OFT did not develop properly in the Nsun5 deletion embryonic heart. We found a misalignment of the aorta and septum defects caused by the delayed fusion of the membraneous ventricular spetum as an OFT development delay. This caused OFT development delay in 27 of 64 (42.2%) Nsun5 –/– mice. Moreover, we also found OFT development delay in 8 of 51 (15.7%) Nsun5 +/– mice. Further functional experiments showed that the loss of Nsun5 function impaired the 5-methylcytosine (m 5 C) modification and translation efficiency of essential cardiac genes. Nsun5 is required for normal OFT morphogenesis and it regulates the m 5 C modification of essential cardiac genes. Our findings suggest the involvement of NSUN5 in the pathogenesis of TOF.
Type of Medium:
Online Resource
ISSN:
2296-634X
DOI:
10.3389/fcell.2021.623394
DOI:
10.3389/fcell.2021.623394.s001
DOI:
10.3389/fcell.2021.623394.s002
DOI:
10.3389/fcell.2021.623394.s003
DOI:
10.3389/fcell.2021.623394.s004
DOI:
10.3389/fcell.2021.623394.s005
DOI:
10.3389/fcell.2021.623394.s006
DOI:
10.3389/fcell.2021.623394.s007
DOI:
10.3389/fcell.2021.623394.s008
DOI:
10.3389/fcell.2021.623394.s009
DOI:
10.3389/fcell.2021.623394.s010
DOI:
10.3389/fcell.2021.623394.s011
DOI:
10.3389/fcell.2021.623394.s012
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2737824-X
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