In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2016-04-25)
Abstract:
Klotho, a protein mainly expressed in kidney and cerebral choroid plexus, is a powerful regulator of 1,25(OH) 2 D 3 formation. Klotho-deficient mice ( kl/kl ) suffer from excessive plasma 1,25(OH) 2 D 3 -, Ca 2+ - and phosphate-concentrations, leading to severe soft tissue calcification and accelerated aging. NH 4 Cl treatment prevents tissue calcification and premature ageing without affecting 1,25(OH) 2 D 3 -formation. The present study explored the impact of excessive 1,25(OH) 2 D 3 formation in NH 4 Cl-treated kl/kl- mice on behavior. To this end kl/kl -mice and wild-type mice were treated with NH 4 Cl and either control diet or vitamin D deficient diet (LVD). As a result, plasma 1,25(OH) 2 D 3 -, Ca 2+ - and phosphate-concentrations were significantly higher in untreated and in NH 4 Cl-treated kl/kl- mice than in wild-type mice, a difference abrogated by LVD. In each, open field, dark-light box, and O-maze NH 4 Cl-treated kl/kl- mice showed significantly higher exploratory behavior than untreated wild-type mice, a difference abrogated by LVD. The time of floating in the forced swimming test was significantly shorter in NH 4 Cl treated kl/kl- mice compared to untreated wild-type mice and to kl/kl -mice on LVD. In wild-type animals, NH 4 Cl treatment did not significantly alter 1,25(OH) 2 D 3 , calcium and phosphate concentrations or exploratory behavior. In conclusion, the excessive 1,25(OH) 2 D 3 formation in klotho-hypomorphic mice has a profound effect on murine behavior.
Type of Medium:
Online Resource
ISSN:
2045-2322
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2016
detail.hit.zdb_id:
2615211-3
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