In:
FEBS Letters, Wiley, Vol. 259, No. 1 ( 1989-12-18), p. 75-78
Abstract:
The synthetic peptide, Asp‐Asp‐Asp‐Glu‐Glu‐Ser‐Ile‐Thr‐Arg‐Arg, derived from the phosphorylation site of casein kinase‐1 (CK‐1) in β‐casein A 2 , is readily phosphorylated by CK‐1, but not by casein kinase‐2 (CK‐2), cyclic AMP‐dependent protein kinase, protein kinsae C, phosphorylase kinase and protein kinase F a . Phosphorylation by CK‐1 occurs only at Ser‐6, Thr‐8 being unaffected. The K m for the peptide is higher (1 mM) than for β‐casein A 2 (40 μM), while the V max is quite comparable. This is the first synthetic peptide substrate for CK‐1 described so far, and can be used for the rapid and specific estimation of CK‐1 activity in crude extracts.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(89)81498-X
Language:
English
Publisher:
Wiley
Publication Date:
1989
detail.hit.zdb_id:
1460391-3
SSG:
12
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