In:
Applied Spectroscopy, SAGE Publications, Vol. 66, No. 1 ( 2012-01), p. 60-65
Abstract:
Linear discriminant analysis (LDA) of single-cell fluorescence excitation spectra (λ em =680 nm) for five species of marine phytoplankton was used to determine whether intra-species variation among single cells precluded discrimination among species. Single-cell spectra were recorded in an optical trap with a custom-built spectral fluorometer. For nitrogen (N)- replete cells, separation of all five species ( Emiliania huxleyi, a coccolithophore, Thalassiosira pseudonana, a diatom, Dunaliella tertiolecta, a chlorophyte, Amphidinium carterae, a dinoflagellate, and Rhodomonas salina, a cryptophyte) was possible using only a portion of the excitation spectra (570–610 nm). This wavelength region gave perfect classification of species with a minimum Fisher ratio of 62. For four species ( E. huxleyi, T. pseudonana, D. tertiolecta, and A. carterae), variations in fluorescence excitation spectra as cells were starved of N did not impact the classification process adversely within the chosen spectral window. R. salina cells grown with and without N showed significant differences in their fluorescence excitation spectra but could still be classified if a different spectral window (490–570 nm) was used. Overall, we conclude that intra-species variation among single-cell fluorescence excitation spectra does not preclude discrimination among species.
Type of Medium:
Online Resource
ISSN:
0003-7028
,
1943-3530
Language:
English
Publisher:
SAGE Publications
Publication Date:
2012
detail.hit.zdb_id:
1474251-2
SSG:
11
Permalink
