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  • Frontiers Media SA  (9)
  • Liu, Yu  (9)
  • 1
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    Online Resource
    Comparative analysis of four types of mesoscale eddies in the North Pacific Subtropical Countercurrent region - part II seasonal variation
    Frontiers Media SA ; 2023
    In:  Frontiers in Marine Science Vol. 10 ( 2023-1-24)
    Details
    In: Frontiers in Marine Science, Frontiers Media SA, Vol. 10 ( 2023-1-24)
    Abstract: The North Pacific Subtropical Countercurrent area (STCC) is high in mesoscale eddy activities. According to the rotation direction of the eddy flow field and the sign of temperature anomaly within the eddy, they can be divided into four categories: cyclonic cold-core eddy (CCE), anticyclonic warm-core eddy (AWE), cyclonic warm-core eddy (CWE) and anticyclonic cold-core eddy (ACE). CCE and AWE are called normal eddies, and CWE and ACE are named abnormal eddies. Based on the OFES data and vector geometry automatic detection method, we find that at the sea surface, the maximum monthly number of the CCE, AWE, CWE, and ACE occurs in December (765.70 ± 52.05), January (688.20 ± 82.53), August (373.40 ± 43.09) and August (533.00 ± 56.92), respectively. The number of normal eddies is more in winter and spring, and less in summer and autumn, while abnormal eddies have the opposite distribution. The maximum rotation velocity of the four types of eddies appears in June (11.71 ± 0.75 cm/s), June (12.24 ± 0.86 cm/s), May (10.63 ± 0.99 cm/s) and June (9.97 ± 0.91 cm/s), which is fast in winter and spring. The moving speed of the four types of eddies is almost similar (about 10 ~ 11 cm/s). The amplitude of normal and abnormal eddies is both high in summer and autumn, and low in winter and spring, with larger amplitudes in normal than abnormal eddies. The eccentricity (defined as the eccentricity of the ellipse obtained by fitting the eddy boundary) of the four types of eddies is also close to each other, and their variation ranges from 0.7 to 0.8, with no apparent seasonal variation. The vertical penetration depth, which has no significant seasonal difference, is 675.13 ± 67.50 m in cyclonic eddies (CCE and CWE), which is deeper than that 622.32 ± 81.85 m in anticyclonic eddies (ACE and AWE). In addition, increasing the defined temperature threshold for abnormal eddies can significantly reduce their numbers but does not change their seasonal variation trend.
    Type of Medium: Online Resource
    ISSN: 2296-7745
    URL: Article
    DOI: 10.3389/fmars.2023.1121731
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
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  • 2
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    DataSheet_1.docx
    Frontiers Media SA ; 2022
    In:  Frontiers in Marine Science Vol. 9 ( 2022-9-2)
    Details
    In: Frontiers in Marine Science, Frontiers Media SA, Vol. 9 ( 2022-9-2)
    Abstract: The North Pacific Subtropical Countercurrent (STCC) region has high mesoscale eddy activities due to its complex circulation structure. This study divides these mesoscale eddies into four types: cyclonic cold-core eddy (CCE), anticyclonic warm-core eddy (AWE), cyclonic warm-core eddy (CWE), and anticyclonic cold-core eddy (ACE) according to the rotation direction of the eddy flow field and the sign of average temperature anomaly within the eddy after spatial high-pass filtering. CCE and ACE are called normal eddies, while CWE and ACE are named abnormal eddies. Using eddy-resolving model data (OFES), this work finds that the abnormal eddy phenomenon mainly occurs in the ocean’s upper layer. The eddy number proportion for CCEs, AWEs, CWEs, and ACEs at the sea surface is 35.60, 32.08, 12.95, and 19.37%. The corresponding average radius is 79.14 ± 3.7, 83.34 ± 3.75, 73.74 ± 4.14, and 79.46 ± 3.89 km, respectively. Each type of eddy’s average amplitude is about 3 cm. Regarding the eddy average eccentricity, the four types of eddies have very close eccentricities, with a range of 0.73 ~ 0.76. If the types of eddies are not distinguished, the eddies generated north of 21°N tend to move southward, while eddies generated south of that latitude tend to move northward. The depth of CCEs, AWEs, CWEs, and ACEs with average eddy nonlinearity larger than one is concentrated in the ocean’s upper layer at 109.0, 116.0, 159.0, and 52.0 m, respectively. This study deepens the understanding of the spatial distribution characteristics of mesoscale eddies in the STCC region.
    Type of Medium: Online Resource
    ISSN: 2296-7745
    URL: Article
    URL: Article
    DOI: 10.3389/fmars.2022.1004300
    DOI: 10.3389/fmars.2022.1004300.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
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  • 3
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    Presentation_1.pdf
    Frontiers Media SA ; 2022
    In:  Frontiers in Marine Science Vol. 9 ( 2022-8-26)
    Details
    In: Frontiers in Marine Science, Frontiers Media SA, Vol. 9 ( 2022-8-26)
    Abstract: Oceanic mesoscale cyclonic (anticyclonic) eddies usually have cold (warm) cores and counterclockwise (clockwise) flow fields in the Northern Hemisphere. However, “abnormal” cyclonic (anticyclonic) eddies with warm (cold) cores and counterclockwise (clockwise) flow fields have recently been identified in the Kuroshio-Oyashio Extension (KOE) region. Here, traditional cyclonic cold-core eddies (CCEs) and anticyclonic warm-core eddies (AWEs) are termed normal eddies, and cyclonic warm-core eddies (CWEs) and anticyclonic cold-core eddies (ACEs) are called abnormal eddies. Applying a vector geometry-based automatic eddy detection method to the Ocean General Circulation Model for the Earth Simulator reanalysis data (OFES), a three-dimensional eddy dataset is obtained and used to quantify the statistical characteristics of these eddies. Results illustrate that the number of CCEs, AWEs, CWEs, and ACEs accounted for 38.46, 36.15, 13.40, and 11.99%, respectively. In the vertical direction, normal eddies are concentrated in the upper 2,000 m, while abnormal eddies are mainly found in the upper 600 m of the ocean. On seasonal scales, normal eddies are more abundant in winter and spring than in summer and autumn, with the opposite trend found for abnormal eddies. Potential density changes modulated by normal eddies are dominated by eddies-induced temperature anomalies, while salinity anomalies dominate the changes modulated by abnormal eddies. This study expands the types of eddies and enriches their understanding in the KOE region.
    Type of Medium: Online Resource
    ISSN: 2296-7745
    URL: Article
    URL: Article
    DOI: 10.3389/fmars.2022.984244
    DOI: 10.3389/fmars.2022.984244.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
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  • 4
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    Development of an Adeno-Associated Virus-Vectored SARS-CoV-2 Vaccine and Its Immunogenicity in Mice
    Frontiers Media SA ; 2022
    In:  Frontiers in Cellular and Infection Microbiology Vol. 12 ( 2022-3-3)
    Details
    In: Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 12 ( 2022-3-3)
    Abstract: Owing to the outbreak of the novel coronavirus (SARS-CoV-2) worldwide at the end of 2019, the development of a SARS-CoV-2 vaccine became an urgent need. In this study, we developed a type 9 adeno-associated virus vectored vaccine candidate expressing a dimeric receptor binding domain (RBD) of the SARS-CoV-2 spike protein (S protein) and evaluated its immunogenicity in a murine model. The vaccine candidate, named AAV9-RBD virus, was constructed by inserting a signal peptide to the N-terminus of two copies of RBD, spaced by a linker, into the genome of a type 9 adeno-associated virus. In vitro assays showed that HeLa cells infected by the recombinant AAV virus expressed high levels of the recombinant RBD protein, mostly found in the cell culture supernatant. The recombinant AAV9-RBD virus was cultured and purified. The genome titer of the purified recombinant AAV9-RBD virus was determined to be 2.4 × 10 13 genome copies/mL (GC/mL) by Q-PCR. Balb/c mice were immunized with the virus by intramuscular injection or nasal drip administration. Eight weeks after immunization, neutralizing antibodies against the new coronavirus pseudovirus were detected in the sera of all mice; the mean neutralizing antibody EC 50 values were 517.7 ± 292.1 (n=10) and 682.8 ± 454.0 (n=10) in the intramuscular injection group and nasal drip group, respectively. The results of this study showed that the recombinant AAV9-RBD virus may be used for the development of a SARS-CoV-2 vaccine.
    Type of Medium: Online Resource
    ISSN: 2235-2988
    URL: Article
    DOI: 10.3389/fcimb.2022.802147
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2619676-1
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  • 5
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    Baicalin Protects Vascular Tight Junctions in Piglets During Glaesserella parasuis Infection
    Frontiers Media SA ; 2021
    In:  Frontiers in Veterinary Science Vol. 8 ( 2021-6-25)
    Details
    In: Frontiers in Veterinary Science, Frontiers Media SA, Vol. 8 ( 2021-6-25)
    Abstract: Glaesserella parasuis ( G. parasuis ) can cause Glässer's disease and severely affect swine industry worldwide. This study is an attempt to address the issue of the capability of G. parasuis to damage the vascular barrier and the effects of baicalin on vascular tight junctions (TJ) in order to investigate the interactions between the pathogen and the porcine vascular endothelium. Piglets were challenged with G. parasuis and treated with or without baicalin. The expressions of vascular TJ genes were examined using RT-PCR. The distribution patterns of TJ proteins were detected by immunofluorescence. The involved signaling pathways were determined by Western blot assays on related proteins. G. parasuis can downregulate TJ expression and disrupt the distribution of TJ proteins. Baicalin can alleviate the downregulation of vascular TJ mRNA, maintain the distribution, and prevent the abnormalities of TJ. These results provide ample evidence that baicalin has the capacity to protect vascular TJ damaged by G. parasuis through inhibiting PKC and MLCK/MLC pathway activation. As a result, baicalin is a promising candidate for application as a natural agent for the prevention and control of G. parasuis infection.
    Type of Medium: Online Resource
    ISSN: 2297-1769
    URL: Article
    DOI: 10.3389/fvets.2021.671936
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2834243-4
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  • 6
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    Metagenomic Sequencing Analysis of the Effects of Colistin Sulfate on the Pig Gut Microbiome
    Frontiers Media SA ; 2021
    In:  Frontiers in Veterinary Science Vol. 8 ( 2021-7-2)
    Details
    In: Frontiers in Veterinary Science, Frontiers Media SA, Vol. 8 ( 2021-7-2)
    Abstract: The gut microbiome plays important roles in maintaining host health, and inappropriate use of antibiotics can cause imbalance, which may contribute to serious disease. However, despite its promise, using metagenomic sequencing to explore the effects of colistin on gut microbiome composition in pig has not been reported. Herein, we evaluated the roles of colistin in gut microbiome modulation in pigs. Metagenomic analysis demonstrated that overall microbial diversity was higher in the colistin group compared with the control group. Antibiotic Resistance Genes Database analysis demonstrated that following colistin treatment, expression levels of tsnr, ant6ia, tetq, oleb, norm, ant3ia , and mexh were significantly upregulated, indicating that colistin may induce transformation of antibiotic resistance genes. Colistin also affected the microbiome distribution patterns at both genus and phylum levels. In addition, at the species level, colistin significantly reduced the abundance of Prevotella copri, Phascolarctobacterium succinatutens , and Prevotella stercorea and enhanced the abundance of Treponema succinifaciens and Acidaminococcus fermentans compared to the control group. Gene Ontology analysis demonstrated that following treatment with colistin, metabolic process, cellular process, and single-organism process were the dominant affected terms. Kyoto Encyclopedia of Genes and Genomes analysis showed that oxidative phosphorylation, protein processing in endoplasmic reticulum, various types of N-glycan biosynthesis, protein processing in endoplasmic reticulum, pathogenic Escherichia coli infection, and mitogen-activated protein kinase signaling pathway–yeast were the dominant signaling pathways in the colistin group. Overall, our results suggested that colistin affects microbial diversity and may modulate gut microbiome composition in pig, potentially providing novel strategy or antibiotic rationalization pertinent to human and animal health.
    Type of Medium: Online Resource
    ISSN: 2297-1769
    URL: Article
    DOI: 10.3389/fvets.2021.663820
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
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  • 7
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    Data_Sheet_9.PDF
    Frontiers Media SA ; 2019
    In:  Frontiers in Microbiology Vol. 10 ( 2019-11-19)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 10 ( 2019-11-19)
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2019.02686
    DOI: 10.3389/fmicb.2019.02686.s001
    DOI: 10.3389/fmicb.2019.02686.s002
    DOI: 10.3389/fmicb.2019.02686.s003
    DOI: 10.3389/fmicb.2019.02686.s004
    DOI: 10.3389/fmicb.2019.02686.s005
    DOI: 10.3389/fmicb.2019.02686.s006
    DOI: 10.3389/fmicb.2019.02686.s007
    DOI: 10.3389/fmicb.2019.02686.s008
    DOI: 10.3389/fmicb.2019.02686.s009
    DOI: 10.3389/fmicb.2019.02686.s010
    DOI: 10.3389/fmicb.2019.02686.s011
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2019
    detail.hit.zdb_id: 2587354-4
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  • 8
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    Table_1.doc
    Frontiers Media SA ; 2021
    In:  Frontiers in Oncology Vol. 11 ( 2021-7-1)
    Details
    In: Frontiers in Oncology, Frontiers Media SA, Vol. 11 ( 2021-7-1)
    Abstract: The lack of cytopathologists delays the advancement of screening for endometrial cancer. It was urgent to develop a new dye for rapid diagnosis. Our study aimed to synthesize a targeted folate receptor-α near-infrared (NIR) fluorescent agent, folic acid-zwitterionic NIR fluorophore (ZW-FA), and explore the feasibility for screening of endometrial cancer and precancerous change. Folic acid was conjugated with zwitterionic NIR fluorophore. The preparation of ZW-FA was validated by 1 H NMR, mass spectrometric, ultraviolet spectra and fluorescence spectra. ZW-FA was incubated with endometrial cytology samples obtained from patients who underwent dilation and curettage or total hysterectomy. Diagnostic utility was calculated by applying laser confocal microscope, Image-J and statistical models, such as enumeration, receiver operating characteristic curve, logistic regression, support vector machine and decision tree were used. The purity of ZW-FA was & gt; 95% determined by 1 H NMR. ZW-FA had the strongest absorption peak at 633 nm in ultraviolet spectra. Photostability of ZW-FA was over 8 hours. In clinical validation, a total of 92 patients were enrolled. The cut-off value of ZW-FA was 49 in enumeration, which was used to distinguish the type of samples. Indicators about diagnostic utility are as follows: sensitivity 90.77%, specificity 62.96%, false-positive rate 37.04%, false-negative rate 9.23%, positive predictive value 85.51% and negative predictive value 73.91%. The samples processed by ZW-FA did not affect further Hematoxylin-Eosin staining and pathological diagnosis. It was an effective cytologic strategy for in vitro diagnosis of endometrial cancer and precancerous change by using ZW-FA. Clinical Trial Registration http://www.chictr.org.cn/index.aspx , identifier ChiCTR1800020123.
    Type of Medium: Online Resource
    ISSN: 2234-943X
    URL: Article
    URL: Article
    DOI: 10.3389/fonc.2021.713583
    DOI: 10.3389/fonc.2021.713583.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2649216-7
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  • 9
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    Data_Sheet_1.pdf
    Frontiers Media SA ; 2020
    In:  Frontiers in Microbiology Vol. 11 ( 2020-6-30)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 11 ( 2020-6-30)
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2020.01324
    DOI: 10.3389/fmicb.2020.01324.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2587354-4
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