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  • SAGE Publications  (8)
  • Liu, An  (8)
  • 1
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    Online Resource
    Differential Expression of PAI-RBP1, C1orf142, and COTL1 in Non–Small Cell Lung Cancer Cell Lines with Different Tumor Metastatic Potential
    SAGE Publications ; 2012
    In:  Journal of Investigative Medicine Vol. 60, No. 4 ( 2012-04), p. 689-694
    Details
    In: Journal of Investigative Medicine, SAGE Publications, Vol. 60, No. 4 ( 2012-04), p. 689-694
    Abstract: Human non–small cell lung cancer (NSCLC) is one of the most common malignancies in the modern world. Its recurrence is mainly due to its ability to invade and metastasize. However, the precise mechanism for tumor development and metastasis is still not fully understood. To shed light on the development of lung cancer, the human giant cell lung carcinoma cell lines 95D with high metastatic potential and 95C with low metastatic potential were selected in this study. The 2 cell lines originated from the same parental cell and share a similar genetic background. In the current study, we identified 3 differentially expressed proteins in 95C and 95D cell lines, namely, PAI-RBP1, C1orf142, and COTL1, by using 2-dimensional electrophoresis proteomics analysis. We found that PAI-RBP1 and C1orf142 expression levels were higher in 95D than in 95C cells, whereas COTL1 expression level was lower in 95D when compared to 95C cells. We also confirmed these results by reverse transcription–polymerase chain reaction and immunoblotting analyses. The messenger RNA and protein levels of PAI-RBP1 and C1orf142 were much higher in 95D than in 95C cells, and COTL1 expression level was lower in 95D than in 95C cells. The PAI-RBP1 expression was assessed by immunohistochemistry in 70 NSCLC and 7 normal lung tissue samples from patients. PAI-RBP1 expression level was higher in tumor tissues (positive staining in 87.1% of cases [61/70]) than in normal tissues (positive staining in 14.3% of cases [1/7] ). In conclusion, by studying protein expression in NSCLC cell lines with high and low metastasis as well as in human lung cancer tissues, we have identified 3 proteins, namely, PAI-RBP1, C1orf142, and COTL1, which were differentially expressed in NSCLC cell lines with different metastatic potential. In addition, we also found that PAI-RBP1 might contribute to NSCLC development.
    Type of Medium: Online Resource
    ISSN: 1081-5589 , 1708-8267
    URL: Article
    DOI: 10.2310/JIM.0b013e31824963b6
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2012
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  • 2
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    Three-dimensional printing akermanite porous scaffolds for load-bearing bone defect repair: An investigation of osteogenic capability and mechanical evolution
    SAGE Publications ; 2016
    In:  Journal of Biomaterials Applications Vol. 31, No. 5 ( 2016-11), p. 650-660
    Details
    In: Journal of Biomaterials Applications, SAGE Publications, Vol. 31, No. 5 ( 2016-11), p. 650-660
    Abstract: Some Ca–Mg-silicate ceramics have been widely investigated to be highly bioactive and biodegradable, whereas their osteogenic potential and especially biomechanical response in the early stage in vivo are scarcely demonstrated. Herein, the osteogenesis capacity and mechanical evolution of the akermanite (Ca 2 MgSi 2 O 7 ) porous materials manufactured by ceramic ink writing three-dimensional printing technique were investigated systematically in a critical size femur defect model, in comparison with the clinically available β-tricalcium phosphate porous bioceramic. Such three-dimensional printed akermanite scaffolds possess fully interconnected pores of ∼280 × 280 µm in size and over 50% porosity with appreciable compressive strength (∼71 MPa), that is 7-fold higher than that of the β-tricalcium phosphate porous bioceramics (∼10 MPa). After 6 weeks and 12 weeks of implantation, the percentage of newly formed bone and more new bone was observed in the akermanite group as compared with the β-tricalcium phosphate group ( p  〈  0.01). Moreover, significant higher mRNA expressions of osteogenic genes were detected in the akermanite group by PCR analysis ( p  〈  0.01). The in vivo mechanical strength decreased during the process of implantation, but maintained a relative high level (∼14 MPa) which was still higher than that of the host cancellous bone (5–10 MPa) at 12 weeks post-implantation. On the contrary, the β-tricalcium phosphate scaffold always exhibited a very low mechanical strength (∼8 MPa). These results suggest that the three-dimensional printed akermanite scaffolds are promising for the bone tissue regeneration and repair of load-bearing bone defects.
    Type of Medium: Online Resource
    ISSN: 0885-3282 , 1530-8022
    URL: Article
    DOI: 10.1177/0885328216664839
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2072559-0
    SSG: 12
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  • 3
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    Mir-142-3p Regulates Inflammatory Response by Contributing to Increased TNF-α in Chronic Rhinosinusitis With Nasal Polyposis
    SAGE Publications ; 2021
    In:  Ear, Nose & Throat Journal Vol. 100, No. 1 ( 2021-01), p. NP50-NP56
    Details
    In: Ear, Nose & Throat Journal, SAGE Publications, Vol. 100, No. 1 ( 2021-01), p. NP50-NP56
    Abstract: Previous studies suggested that microRNAs played an important role in the progression of inflammation and remodeling of chronic rhinosinusitis with nasal polyposis. However, the abnormal expression of microRNAs and regulation cytokine expression in nasal polyposis are not clear. Method: The miR-142-3p and tumor necrosis factor α (TNF-α) expression levels in chronic rhinosinusitis with nasal polyposis were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The miR-142-3p and TNF-α levels in human nasal epithelial cells (HNEpC) after stimulation by lipopolysaccharide (LPS) were detected by qRT-PCR. Moreover, HNEpCs were transfected by miR-142-3p mimics or inhibitor or cotransfected with si-TNF-α to evaluate the regulation of miR-142-3p on TNF-α which affects the production of inflammatory factors. Results: The miR-142-3p and TNF-α were significantly higher in nasal mucosa of chronic rhinosinusitis with polyps patients compared to normal human. MiR-142-3p and TNF-α expression levels were increased after LPS stimulation in a dose- and time-dependent manner. Knockdown of miR-142-3p in HNEpCs downregulated TNF-α expression at both messenger RNA and protein levels. Conclusions: It is indicated that miR-142-3p may participate in the regulation of the body’s inflammatory response through the LPS-TLR-TNF-α signaling pathway in chronic rhinosinusitis with nasal polyposis.
    Type of Medium: Online Resource
    ISSN: 0145-5613 , 1942-7522
    URL: Article
    DOI: 10.1177/0145561319847972
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2067528-8
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  • 4
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    Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model
    SAGE Publications ; 2020
    In:  Molecular Pain Vol. 16 ( 2020-01), p. 174480691990071-
    Details
    In: Molecular Pain, SAGE Publications, Vol. 16 ( 2020-01), p. 174480691990071-
    Type of Medium: Online Resource
    ISSN: 1744-8069 , 1744-8069
    URL: Article
    DOI: 10.1177/1744806919900717
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2174252-2
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  • 5
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    Monitoring the quality of conduct of clinical trials: a survey of current practices
    SAGE Publications ; 2011
    In:  Clinical Trials Vol. 8, No. 3 ( 2011-06), p. 342-349
    Details
    In: Clinical Trials, SAGE Publications, Vol. 8, No. 3 ( 2011-06), p. 342-349
    Abstract: Background There is a little empirical evidence to determine which, if any, monitoring practices best achieve the goals of trial monitoring set forth in ICH E6 under the variable circumstances of different clinical trial settings. Purpose The purpose of this project was to describe current methods of monitoring clinical trials and to explore the rationale for the use of those methods. Methods An electronic survey of known monitoring practices was developed and sent to over 200 organizations involved in conducting clinical research. The survey collected information on institutional demographics, methods of overall study oversight, use of risk-based monitoring and factors that influence assessments of risk, and details on quality assurance and monitoring practices. Results Seventy-nine organizations completed the survey; our analysis included the 65 organizations that indicated they perform clinical trials. Data from the survey indicate that a wide variety of monitoring practices are currently being employed. Eighty-three percent of respondents use centrally available data to evaluate site performance, but only 12% of respondents always or frequently used centralized monitoring to replace on-site visits. Eighty-seven percent of respondents indicated that they always performed on-site visits. This varied by type of organization, with 31% of academic coordinating centers/cooperative groups/government organizations always performing on-site monitoring visits versus 84% of other organizations. The rationale for using a specific monitoring approach does not appear to be based on empirical evidence. Fifty-four percent of respondents stated that ‘usual practice’ determined the frequency with which they conducted on-site monitoring visits. Limitations The overall response rate to our survey was only 30%; thus, we may not have captured the full variance of current monitoring practices, and our responding sample may not be representative. Conclusion These findings underscore the necessity of research to provide an evidence base for monitoring practice.
    Type of Medium: Online Resource
    ISSN: 1740-7745 , 1740-7753
    URL: Article
    DOI: 10.1177/1740774511402703
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2011
    detail.hit.zdb_id: 2159773-X
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  • 6
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    Synergistic Effect of Reverse Drilling and Bone Dust on Femoral Tendon-Bone Healing After Anterior Cruciate Ligament Reconstruction in a Rabbit Model
    SAGE Publications ; 2022
    In:  The American Journal of Sports Medicine Vol. 50, No. 14 ( 2022-12), p. 3844-3855
    Details
    In: The American Journal of Sports Medicine, SAGE Publications, Vol. 50, No. 14 ( 2022-12), p. 3844-3855
    Abstract: Anterior cruciate ligament (ACL) injuries and bone tunnel enlargement (BTE) after ACL reconstruction (ACLR) remain frequent issues. Bone dust (BD) produced by tunnel preparation with osteogenic ability and reverse drilling (RD), an easy compaction technique, make it accessible to enhance tendon-bone healing in the clinic. Hypothesis: RD and BD synergistically promote tendon-bone healing by improving peritunnel bone and preventing BTE in femurs. Study Design: Controlled laboratory study. Methods: In total, 96 New Zealand White rabbits underwent ACLR. The semitendinosus tendon was freed before medial parapatellar arthrotomy. After the native ACL was transected, bone tunnels were prepared through the footprint of the native ACL. All animals were randomly assigned to 1 of 4 groups according to different tunnel preparation methods: group 1 (irrigation after extraction drilling [ED]; control group), group 2 (irrigation after RD), group 3 (no irrigation after ED), and group 4 (no irrigation after RD). BD was harvested by irrigating tunnels and was characterized by morphology and size. The specimens underwent microarchitectural, histological, and biomechanical evaluations at 4, 8, and 12 weeks postoperatively. Results: Micro–computed tomography demonstrated more peritunnel bone and less BTE in the femurs of group 4 compared with the other groups. Histologically, BD possessed osteogenic activity in bone tunnels postoperatively. Meanwhile, group 4 regenerated a higher amount of the tendon-bone interface and more peritunnel bone than group 1. Biomechanically, group 4 showed higher failure loads and stiffness than group 1. However, peritunnel bone loss, active osteoclasts, and significant BTE were found in the femurs of group 1 and group 3 at 12 weeks postoperatively, while no strong correlation was found between BTE and inflammatory cytokines. Scanning electron microscopy and particle size analysis suggested that BD produced by ED and RD had no difference in size. Conclusion: Tendon-bone healing was facilitated by the synergistic effect of RD and BD in femurs. Clinical Relevance: This study provides a more accessible and effective surgical strategy to promote tendon-bone healing after ACLR by increasing peritunnel bone and preventing BTE in femurs.
    Type of Medium: Online Resource
    ISSN: 0363-5465 , 1552-3365
    URL: Article
    DOI: 10.1177/03635465221129267
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2063945-4
    SSG: 31
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  • 7
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    Wireless Sensor Network Security
    SAGE Publications ; 2013
    In:  International Journal of Distributed Sensor Networks Vol. 9, No. 1 ( 2013-01-01), p. 362385-
    Details
    In: International Journal of Distributed Sensor Networks, SAGE Publications, Vol. 9, No. 1 ( 2013-01-01), p. 362385-
    Type of Medium: Online Resource
    ISSN: 1550-1477 , 1550-1477
    URL: Article
    DOI: 10.1155/2013/362385
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2192922-1
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  • 8
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    Estimation of Autoregressive Models with Two Types of Weak Spatial Dependence by Means of the W-Based and the Latent Variables Approach: Evidence from Monte Carlo Simulations
    SAGE Publications ; 2014
    In:  Environment and Planning A: Economy and Space Vol. 46, No. 1 ( 2014-01), p. 186-202
    Details
    In: Environment and Planning A: Economy and Space, SAGE Publications, Vol. 46, No. 1 ( 2014-01), p. 186-202
    Abstract: We evaluate by means of Monte Carlo simulations the W-based spatial autoregressive model and the structural equation model with latent variables (SEM) to handle two types of simultaneously occurring weak spatial dependence: (i) spillover from a hotspot, and (iia) spillover from first-order, queen contiguous neighbours, (iib) inverse-distance-related spatial units, (iic) the first three nearest neighbours. Although it is possible to account for several different types of weak spatial lag dependence by several different weights matrices, the W-based approach usually proceeds on the basis of a single weights matrix that is implicitly assumed to adequately capture all types of spatial dependence. SEM on the other hand has been developed to explicitly distinguish between different types of weak spatial dependence in a model. In addition, whereas conventional higher order spatial econometric models entail specification and parameter space definition problems, SEM spatial dependence models can be routinely specified and estimated. In this paper we briefly discuss the pitfalls of including several types of weak spatial lag dependence (and thus different W matrices) in a standard W-based lag model and how these problems are mitigated by the SEM approach. Furthermore, the single W-based and the SEM approach are compared by means of simulations in terms of bias and root mean squared error (RMSE) for different values of the spatial lag parameters, specifications of the weights matrices, and sample sizes. We also include in the comparison the W-based approach with spatial dependence accounted for by the two weights matrices used to generate the data: That is, the correctly specified model. The simulation results show that compared with the single W-based models, SEM frequently has smaller bias and RMSE. Furthermore, SEM even outperforms the correctly specified W-based models (based on the same two weights matrices used for data generation) in many cases. These trends increase when the values of the spatial lag parameters increase. The dominance of SEM also increases with sample size. Finally, SEM is more stable in terms of both bias and RMSE over various dimensions.
    Type of Medium: Online Resource
    ISSN: 0308-518X , 1472-3409
    URL: Article
    DOI: 10.1068/a45558
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2039728-8
    detail.hit.zdb_id: 750312-X
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