In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 64, No. 8 ( 2012-07-09), p. 1155-1164
Abstract:
The effects of rel-(1S,4aS,7S,8aS)-7-(1-vinyl)-tetradecahydro-1,4a-dimethylphenanthrene-7,8a-carbolactone-1-carboxylic acid (TCCA), a new ent-kaurene diterpene isolated from Croton argyrophylloides, on rat tracheal preparations were investigated. Methods Tracheae were removed and cut into two-cartilage segments that were mounted in organ baths containing Tyrode's solution. Results TCCA reduced the contractions induced by electrical field stimulation, relaxed K+-induced contractions, and inhibited both phasic and tonic components of the K+- and ACh-induced contractions. TCCA reduced the serotonin-induced contraction, abolished that evoked by K+ in the presence of epinephrine, and also reduced the ACh-induced contractions under Ca2+-free conditions. TCCA blocked contractions that depend on divalent cation inflow through voltage-operated Ca2+ channels (VOCCs) and receptor-operated Ca2+ channels (ROCCs), but had greater potency to block VOCC- than ROCC-dependent contractions or contractions induced by ACh in Ca2+-free conditions. TCCA relaxed the phorbol 12,13 dibutyrate (1 µm) induced contraction, but with slight potency. Conclusions TCCA induces an antispasmodic effect through several mechanisms including blockade of either VOCCs (with greater potency) or ROCCs, blockade of IP3-induced Ca2+ release from sarcoplasmic reticulum (with intermediate potency) and reduction of the sensitivity of contractile proteins to Ca2+.
Type of Medium:
Online Resource
ISSN:
2042-7158
,
0022-3573
DOI:
10.1111/j.2042-7158.2012.01494.x
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2012
detail.hit.zdb_id:
2041988-0
detail.hit.zdb_id:
2050532-2
SSG:
15,3
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