In:
Journal of Advances in Biology & Biotechnology, Sciencedomain International, ( 2022-12-12), p. 13-22
Abstract:
Periodontitis is a chronic disease associated with dental biofilm formation by bacteria. The primary clinical signs include plaque formation, gingivitis, gingival bleeding, and attachment loss. P-113, a 12-amino acid antimicrobial peptide, is derived from Histatin 5 (Hst5) secreted by the human parotid and submandibular salivary glands and has been reported to reduce plaque formation, gingivitis, and gingival bleeding. This study aimed to investigate the effect of P-113 on periodontal disease-associated bacteria and the relative abundance of bacteria using real-time polymerase chain reaction (real-time PCR) and Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Saliva samples were collected from participants for analyses. Real-time PCR results showed significant changes in the abundance of the periodontal disease-associated bacteria, Fusobacterium nucleatum and Tannerella forsythia, after the use of the P-113 mouthwash. After culturing in an anaerobic environment, MALDI-TOF MS analysis was performed. The results showed that the relative abundance of Streptococcus mitis decreased and that of beneficial Streptococcus salivarius increased after the use of the P-113 mouthwash. These results indicate the potential of P-113 mouthwash as a treatment that can influence the proportion of pathogens and probiotics to establish equilibrium in the salivary environment.
Type of Medium:
Online Resource
ISSN:
2394-1081
DOI:
10.9734/jabb/2022/v25i9595
Language:
Unknown
Publisher:
Sciencedomain International
Publication Date:
2022
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