In:
FEBS Letters, Wiley, Vol. 199, No. 1 ( 1986-04-07), p. 100-102
Abstract:
The neurotoxin 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) and its deuterated analogues were oxidized to their corresponding dihydropyridinium species (MPDP + ) by preparations of pure human liver MAO B : monoclonal antibody complex to investigate the mechanism of MPTP activation. Lineweaver‐Burk plots of initial reaction rates revealed that the K m,app values for the various deuterated MPTP analogues were similar to those of MPTP. In contrast, V max,app values were substantially decreased by substitution of deuterium for hydrogen on the tetrahydropyridinium ring, especially at C‐6. Deuterium substitution on the N ‐methyl group alone did not significantly reduce V max,app . These studies support the interpretation that oxidation of MPTP at the C‐6 position on the tetrahydropyridine ring is a major rate‐determining step in its biotransformation by MAO B.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(86)81232-7
Language:
English
Publisher:
Wiley
Publication Date:
1986
detail.hit.zdb_id:
1460391-3
SSG:
12
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