In:
Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 57 ( 2005-1-1)
Abstract:
This study examined the effects of molting stage on the growth of primary-cultured cells from the shrimps Macrobrachium nipponense and Penaeus vannamei. Muscle cells in different molting stages were cultured in L-15 medium, supplemented with 1g/l glucose,1g/l MgCl2,1 g/l CaCl2,100 IU/ml penicillin, and 100 μg/ml streptomycin. Results showed that cells cultured in the early postmolt stage grew best. They had an unfolding cell shape, a compact monolayer, a high rate of coverage, and the highest RNA:DNA ratio. Differences among early postmolt, premolt, and intermolt cells were significant (p 〈 0.05).
Type of Medium:
Online Resource
ISSN:
0792-156X
,
0792-156X
Language:
English
Publisher:
SAABRON PRESS
Publication Date:
2005
detail.hit.zdb_id:
291787-7
detail.hit.zdb_id:
2542938-3
SSG:
21,3
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