In:
Journal of Forensic Sciences, Wiley, Vol. 63, No. 6 ( 2018-11), p. 1692-1703
Abstract:
Next‐generation sequencing ( NGS ) has been used to genotype forensic short tandem repeat ( STR ) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25‐plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA . In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping.
Type of Medium:
Online Resource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/jfo.2018.63.issue-6
DOI:
10.1111/1556-4029.13767
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2026357-0
SSG:
2,1
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